##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AhdI Aor51HI ApaI AscI AspEI
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrDI BssHII BstBI BstEII
BstPI BstUI CelII CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FauI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3577 GGTACC G^GTACC
AciI 596 CCGC CCGC(-3/-1)
AclI 3861 AACGTT AA^CGTT
AcyI 215 GACGCC G[GA]^CG[CT]C
AflIII 1609 ACATGT A^C[GA][CT]GT
Alw44I 4018 GTGCAC G^TGCAC
ApaLI 4018 GTGCAC G^TGCAC
AseI 2564 ATTAAT AT^TAAT
AsnI 2564 ATTAAT AT^TAAT
Asp700I 2707 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3577 GGTACC G^GTACC
BbvCI 4049 GCTGAGG CCTCAGC(-5/-2)
BciVI 3300 GGATAC GTATCC(6/5)
BclI 3925 TGATCA T^GATCA
BglI 1211 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 215 GACGCC G[GA]^CG[CT]C
BsaI 3948 GAGACC GGTCTC(1/5)
BsaWI 2860 ACCGGA [AT]^CCGG[AT]
BspLU11I 1609 ACATGT A^CATGT
BsrFI 3098 GCCGGC [GA]^CCGG[CT]
BssSI 4056 CACGAG CACGAG(-5/-1)
BstAPI 2178 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 3098 GCCGGC [GA]^CCGG[CT]
EarI 3014 CTCTTC CTCTTC(1/4)
EcoNI 141 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3635 GATATC GAT^ATC
FbaI 3925 TGATCA T^GATCA
HgaI 215 GACGC GACGC(5/10)
Hin1I 215 GACGCC G[GA]^CG[CT]C
HincII 2660 GTTGAC GT[CT]^[GA]AC
HindII 2660 GTTGAC GT[CT]^[GA]AC
HindIII 62 AAGCTT A^AGCTT
KpnI 3577 GGTACC GGTAC^C
Ksp632I 3014 CTCTTC CTCTTC(1/4)
NaeI 3098 GCCGGC GCC^GGC
NdeI 402 CATATG CA^TATG
NgoMIV 3098 GCCGGC G^CCGGC
NheI 358 GCTAGC G^CTAGC
NspI 1609 ACATGT [GA]CATG^[CT]
PshBI 2564 ATTAAT AT^TAAT
Psp1406I 3861 AACGTT AA^CGTT
SapI 3013 GCTCTTC GCTCTTC(1/4)
ScaI 129 AGTACT AGT^ACT
SexAI 1023 ACCTGGT A^CC[AT]GGT
StuI 2558 AGGCCT AGG^CCT
XbaI 1351 TCTAGA T^CTAGA
XcmI 219 CCAAAGCACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2707 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1465 GTATAC GT^[AC][GT]AC
1484 GTATAC
AocI 1860 CCTTAGG CC^T[ACGT]AGG
3931 CCTGAGG
AspHI 3591 GTGCTC G[AT]GC[AT]^C
4018 GTGCAC
AspI 676 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2125 GACAGAGTC
BbsI 3006 GAAGAC GAAGAC(2/6)
2528 GTCTTC
BglII 530 AGATCT A^GATCT
3703 AGATCT
BpuAI 3006 GAAGAC GAAGAC(2/6)
2528 GTCTTC
BseRI 238 CTCCTC GAGGAG(10/8)
4043 GAGGAG
BsiHKAI 3591 GTGCTC G[AT]GC[AT]^C
4018 GTGCAC
Bst1107I 1465 GTATAC GTA^TAC
1484 GTATAC
BstZ17I 1465 GTATAC GTA^TAC
1484 GTATAC
Bsu36I 1860 CCTTAGG CC^T[ACGT]AGG
3931 CCTGAGG
DraII 728 GGGTCCT [GA]G^G[ACGT]CC[CT]
2945 AGGACCT
DsaI 192 CCATGG C^C[GA][CT]GG
3877 CCATGG
Eco81I 1860 CCTTAGG CC^T[ACGT]AGG
3931 CCTGAGG
EcoO109I 728 GGGTCCT [GA]G^G[ACGT]CC[CT]
2945 AGGACCT
EcoRI 1355 GAATTC G^AATTC
3710 GAATTC
EcoT22I 1744 ATGCAT ATGCA^T
3811 ATGCAT
MaeII 1569 ACGT A^CGT
3862 ACGT
MspA1I 3117 CAGCTG C[AC]G^C[GT]G
3124 CAGCTG
NcoI 192 CCATGG C^CATGG
3877 CCATGG
NsiI 1744 ATGCAT ATGCA^T
3811 ATGCAT
PflFI 676 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2125 GACAGAGTC
PpuMI 728 GGGTCCT [GA]G^G[AT]CC[CT]
2945 AGGACCT
PvuII 3117 CAGCTG CAG^CTG
3124 CAGCTG
SfcI 763 CTGTAG C^T[GA][CT]AG
1113 CTACAG
SmaI 2191 CCCGGG CCC^GGG
4073 CCCGGG
SpeI 1984 ACTAGT A^CTAGT
3647 ACTAGT
TaqI 2002 TCGA T^CGA
2303 TCGA
Tth111I 676 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2125 GACAGAGTC
TthHB8I 2002 TCGA T^CGA
2303 TCGA
XmaCI 2191 CCCGGG C^CCGGG
4073 CCCGGG
XmaI 2191 CCCGGG C^CCGGG
4073 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaI AvaII BalI BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII