##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI Asp718I AspEI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaBI BsiWI BspDI BspHI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV FbaI FseI KpnI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PshAI
Psp1406I PvuI RcaI SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 197 TCCGGA T^CCGGA
AcsI 4550 AAATTC [GA]^AATT[CT]
Alw44I 1882 GTGCAC G^TGCAC
ApaLI 1882 GTGCAC G^TGCAC
ApoI 4550 AAATTC [GA]^AATT[CT]
AseI 4609 ATTAAT AT^TAAT
AsnI 4609 ATTAAT AT^TAAT
AviII 3588 TGCGCA TGC^GCA
BseAI 197 TCCGGA T^CCGGA
BsiEI 4277 CGGCCG CG[GA][CT]^CG
BsmI 2450 GCATTC GAATGC(1/-1)
BspEI 197 TCCGGA T^CCGGA
BstAPI 239 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 911 CGGACCG CG^G[AT]CCG
DraIII 4299 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4277 CGGCCG C^GGCCG
EclXI 4277 CGGCCG C^GGCCG
Eco52I 4277 CGGCCG C^GGCCG
EcoT22I 1787 ATGCAT ATGCA^T
FspI 3588 TGCGCA TGC^GCA
HincII 1281 GTTAAC GT[CT]^[GA]AC
HindII 1281 GTTAAC GT[CT]^[GA]AC
HindIII 1971 AAGCTT A^AGCTT
HpaI 1281 GTTAAC GTT^AAC
MroI 197 TCCGGA T^CCGGA
NcoI 160 CCATGG C^CATGG
NsiI 1787 ATGCAT ATGCA^T
PaeR7I 2495 CTCGAG C^TCGAG
PshBI 4609 ATTAAT AT^TAAT
RsrII 911 CGGACCG CG^G[AT]CCG
ScaI 1136 AGTACT AGT^ACT
SexAI 3672 ACCTGGT A^CC[AT]GGT
Tsp45I 3230 GTCAC ^GT[GC]AC
XhoI 2495 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 680 ACACGT A^C[GA][CT]GT
1564 ACACGT
Aor51HI 23 AGCGCT AGC^GCT
764 AGCGCT
AvrII 3372 CCTAGG C^CTAGG
3384 CCTAGG
BalI 773 TGGCCA TGG^CCA
2675 TGGCCA
BbrPI 681 CACGTG CAC^GTG
1565 CACGTG
BbsI 4070 GAAGAC GAAGAC(2/6)
1552 GTCTTC
BcgI 1040 GCAAATGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1513 GCAGATGAGTCG
BlnI 3372 CCTAGG C^CTAGG
3384 CCTAGG
BpuAI 4070 GAAGAC GAAGAC(2/6)
1552 GTCTTC
BsmBI 2390 CGTCTC CGTCTC(1/5)
2695 CGTCTC
Bsp1407I 431 TGTACA T^GTACA
1666 TGTACA
BsrDI 2826 CATTGC GCAATG(2/0)
328 GCAATG
BsrGI 431 TGTACA T^GTACA
1666 TGTACA
BssSI 2552 CACGAG CACGAG(-5/-1)
3495 CTCGTG
EarI 10 GAAGAG CTCTTC(1/4)
1251 GAAGAG
Eco47III 23 AGCGCT AGC^GCT
764 AGCGCT
HgaI 172 GCGTC GACGC(5/10)
3545 GCGTC
Ksp632I 10 GAAGAG CTCTTC(1/4)
1251 GAAGAG
MluNI 773 TGGCCA TGG^CCA
2675 TGGCCA
MscI 773 TGGCCA TGG^CCA
2675 TGGCCA
NspI 2407 ACATGC [GA]CATG^[CT]
3896 ACATGC
PmaCI 681 CACGTG CAC^GTG
1565 CACGTG
PmlI 681 CACGTG CAC^GTG
1565 CACGTG
PpuMI 803 GGGACCT [GA]G^G[AT]CC[CT]
3023 GGGACCC
PstI 397 CTGCAG CTGCA^G
2357 CTGCAG
SacI 276 GAGCTC GAGCT^C
2315 GAGCTC
SmaI 60 CCCGGG CCC^GGG
3438 CCCGGG
SspBI 431 TGTACA T^GTACA
1666 TGTACA
XcmI 1924 CCAGCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3863 CCAAGCTGGGGGTGG
XmaCI 60 CCCGGG C^CCGGG
3438 CCCGGG
XmaI 60 CCCGGG C^CCGGG
3438 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AspI AvaI AvaII BamHI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI