##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AspI AvrII
BbrPI BclI BfrI BglII BlnI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoRV EcoT22I FbaI HpaI KspI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SfiI
SfuI SmaI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3000 GGTACC G^GTACC
AccI 2546 GTCTAC GT^[AC][GT]AC
AcsI 3188 GAATTC [GA]^AATT[CT]
AflIII 2847 ACGTGT A^C[GA][CT]GT
AhdI 4184 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1294 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3684 AGCGCT AGC^GCT
ApoI 3188 GAATTC [GA]^AATT[CT]
Asp718I 3000 GGTACC G^GTACC
AspEI 4184 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2964 GGATCC G^GATCC
BbeI 2926 GGCGCC GGCGC^C
BcgI 2611 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2846 TACGTG [CT]AC^GT[GA]
BsaBI 3319 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3678 ACCGGA [AT]^CCGG[AT]
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsrDI 1329 GCAATG GCAATG(2/0)
BstAPI 3782 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3457 GGTCACC G^GT[ACGT]ACC
BstPI 3457 GGTCACC G^GT[ACGT]ACC
Bsu36I 1294 CCTGAGG CC^T[ACGT]AGG
DrdI 3287 GACCTCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 11 CGGCCG C^GGCCG
Eam1105I 4184 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 11 CGGCCG C^GGCCG
Eco47III 3684 AGCGCT AGC^GCT
Eco52I 11 CGGCCG C^GGCCG
Eco81I 1294 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3457 GGTCACC G^GT[ACGT]ACC
EcoRI 3188 GAATTC G^AATTC
FseI 1399 GGCCGGCC GGCCGG^CC
HindIII 1623 AAGCTT A^AGCTT
KasI 2926 GGCGCC G^GCGCC
KpnI 3000 GGTACC GGTAC^C
MamI 3319 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 4211 TTAA T^TAA
NarI 2926 GGCGCC GG^CGCC
SapI 1159 GCTCTTC GCTCTTC(1/4)
ScaI 3983 AGTACT AGT^ACT
SfoI 2926 GGCGCC GGC^GCC
SgrAI 2432 CGCCGGTG C[GA]^CCGG[CT]G
StuI 1812 AGGCCT AGG^CCT
Tru9I 4211 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 590 CGCG CG^CG
2588 CGCG
ApaI 2949 GGGCCC GGGCC^C
4061 GGGCCC
Asp700I 2485 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3682 GAAGCGCTTC
AviII 594 TGCGCA TGC^GCA
1044 TGCGCA
BciVI 4096 GTATCC GTATCC(6/5)
4134 GTATCC
BglI 1872 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2068 GCCCGGCTGGC
BsgI 149 GTGCAG GTGCAG(16/14)
3530 GTGCAG
Bsp1407I 450 TGTACA T^GTACA
2331 TGTACA
BsrGI 450 TGTACA T^GTACA
2331 TGTACA
BstUI 590 CGCG CG^CG
2588 CGCG
DraIII 861 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1426 CACCTGGTG
EcoNI 1981 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3115 CCTACAGCAGG
FspI 594 TGCGCA TGC^GCA
1044 TGCGCA
HincII 1963 GTCAAC GT[CT]^[GA]AC
2470 GTTGAC
HindII 1963 GTCAAC GT[CT]^[GA]AC
2470 GTTGAC
MaeII 1344 ACGT A^CGT
2847 ACGT
MvnI 590 CGCG CG^CG
2588 CGCG
PleI 2819 GACTC GAGTC(4/5)
788 GAGTC
SbfI 850 CCTGCAGG CCTGCA^GG
1405 CCTGCAGG
Sse8387I 850 CCTGCAGG CCTGCA^GG
1405 CCTGCAGG
SspBI 450 TGTACA T^GTACA
2331 TGTACA
TfiI 1230 GATTC G^A[AT]TC
2883 GAATC
XmnI 2485 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3682 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII