##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AhdI Aor51HI ApoI AscI
Asp718I AspEI AspI AviII BciVI
BclI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NspV PacI PaeR7I
PflFI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 1270 CTTAAG C^TTAAG
AgeI 3969 ACCGGT A^CCGGT
Alw44I 745 GTGCAC G^TGCAC
ApaLI 745 GTGCAC G^TGCAC
AseI 655 ATTAAT AT^TAAT
AsnI 655 ATTAAT AT^TAAT
Asp700I 2948 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1068 CCTAGG C^CTAGG
BbrPI 456 CACGTG CAC^GTG
BfrI 1270 CTTAAG C^TTAAG
BglI 2627 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1068 CCTAGG C^CTAGG
BlpI 3391 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3391 GCTCAGC GC^T[ACGT]AGC
BsaAI 456 CACGTG [CT]AC^GT[GA]
BsmI 872 GCATTC GAATGC(1/-1)
Bsp1407I 595 TGTACA T^GTACA
BsrDI 2740 GCAATG GCAATG(2/0)
BsrGI 595 TGTACA T^GTACA
CelII 3391 GCTCAGC GC^T[ACGT]AGC
DraIII 2566 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4014 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1748 ATGCAT ATGCA^T
NruI 3166 TCGCGA TCG^CGA
NsiI 1748 ATGCAT ATGCA^T
PinAI 3969 ACCGGT A^CCGGT
PmaCI 456 CACGTG CAC^GTG
PmlI 456 CACGTG CAC^GTG
PshBI 655 ATTAAT AT^TAAT
SgrAI 3968 CACCGGTG C[GA]^CCGG[CT]G
SspBI 595 TGTACA T^GTACA
SwaI 3802 ATTTAAAT ATTT^AAAT
Tsp509I 1641 AATT ^AATT
XcmI 3630 CCAAGCCAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2948 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 2590 GGCGCC G[GA]^CG[CT]C
2682 GGCGCC
AflIII 455 ACACGT A^C[GA][CT]GT
457 ACGTGT
AocI 262 CCTAAGG CC^T[ACGT]AGG
2346 CCTGAGG
BamHI 1072 GGATCC G^GATCC
2028 GGATCC
BbeI 2590 GGCGCC GGCGC^C
2682 GGCGCC
BcgI 2631 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2524 GCAGCATGATCG
BsaHI 2590 GGCGCC G[GA]^CG[CT]C
2682 GGCGCC
BsaI 3665 GAGACC GGTCTC(1/5)
2240 GGTCTC
BsaWI 1233 TCCGGT [AT]^CCGG[AT]
3969 ACCGGT
BsgI 463 GTGCAG GTGCAG(16/14)
2574 GTGCAG
BsmBI 1821 GAGACG CGTCTC(1/5)
2535 GAGACG
BsrFI 615 ACCGGC [GA]^CCGG[CT]
3969 ACCGGT
BstAPI 1095 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1391 GCAGAGACTGC
Bsu36I 262 CCTAAGG CC^T[ACGT]AGG
2346 CCTGAGG
Cfr10I 615 ACCGGC [GA]^CCGG[CT]
3969 ACCGGT
Eco81I 262 CCTAAGG CC^T[ACGT]AGG
2346 CCTGAGG
HaeII 2590 GGCGCC [GA]GCGC^[CT]
2682 GGCGCC
HgaI 2597 GACGC GACGC(5/10)
3238 GCGTC
Hin1I 2590 GGCGCC G[GA]^CG[CT]C
2682 GGCGCC
HincII 3189 GTCAAC GT[CT]^[GA]AC
3684 GTTGAC
HindII 3189 GTCAAC GT[CT]^[GA]AC
3684 GTTGAC
KasI 2590 GGCGCC G^GCGCC
2682 GGCGCC
KspI 2923 CCGCGG CCGC^GG
3669 CCGCGG
MaeII 457 ACGT A^CGT
3828 ACGT
NarI 2590 GGCGCC GG^CGCC
2682 GGCGCC
NcoI 489 CCATGG C^CATGG
942 CCATGG
NspI 2701 GCATGC [GA]CATG^[CT]
3177 GCATGC
SacII 2923 CCGCGG CCGC^GG
3669 CCGCGG
SfoI 2590 GGCGCC GGC^GCC
2682 GGCGCC
SphI 2701 GCATGC GCATG^C
3177 GCATGC
SspI 1220 AATATT AAT^ATT
3884 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI