##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI Aor51HI AscI AseI
AsnI AviII BamHI BciVI BglII
BlpI Bpu1102I BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI FspI MfeI
MflI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 80 GGTACC G^GTACC
AflII 3243 CTTAAG C^TTAAG
AgeI 3034 ACCGGT A^CCGGT
Asp718I 80 GGTACC G^GTACC
BcgI 4343 CGAGGGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3243 CTTAAG C^TTAAG
BglI 2748 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 777 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3034 ACCGGT [AT]^CCGG[AT]
DraI 4006 TTTAAA TTT^AAA
EcoRI 2561 GAATTC G^AATTC
HpaI 1556 GTTAAC GTT^AAC
KpnI 80 GGTACC GGTAC^C
KspI 990 CCGCGG CCGC^GG
MamI 777 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PinAI 3034 ACCGGT A^CCGGT
SacII 990 CCGCGG CCGC^GG
SapI 3199 GCTCTTC GCTCTTC(1/4)
SexAI 1162 ACCTGGT A^CC[AT]GGT
SfiI 2747 GGCCTTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 615 GACGTC GACGT^C
769 GACGTC
AhdI 2146 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2527 GACAGGATGTC
AlwI 2036 GATCC GGATC(4/5)
4569 GGATC
AocI 2886 CCTCAGG CC^T[ACGT]AGG
3188 CCTAAGG
Asp700I 1908 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3107 GAAGTCTTTC
AspEI 2146 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2527 GACAGGATGTC
AvrII 2763 CCTAGG C^CTAGG
4433 CCTAGG
BbeI 191 GGCGCC GGCGC^C
2630 GGCGCC
BbrPI 2111 CACGTG CAC^GTG
3018 CACGTG
BlnI 2763 CCTAGG C^CTAGG
4433 CCTAGG
BsiEI 652 CGACCG CG[GA][CT]^CG
3523 CGGTCG
BsrDI 75 GCAATG GCAATG(2/0)
1371 GCAATG
BsrFI 3034 ACCGGT [GA]^CCGG[CT]
4353 GCCGGT
BstAPI 2215 GCATGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2648 GCATTTATTGC
Bsu36I 2886 CCTCAGG CC^T[ACGT]AGG
3188 CCTAAGG
Cfr10I 3034 ACCGGT [GA]^CCGG[CT]
4353 GCCGGT
DraIII 8 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1343 CACCCTGTG
DrdI 3273 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3434 GACAGGACAGTC
Eam1105I 2146 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2527 GACAGGATGTC
Eco81I 2886 CCTCAGG CC^T[ACGT]AGG
3188 CCTAAGG
EcoNI 872 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2758 CCTGTCCTAGG
HaeII 191 GGCGCC [GA]GCGC^[CT]
2630 GGCGCC
HindIII 2204 AAGCTT A^AGCTT
4138 AAGCTT
KasI 191 GGCGCC G^GCGCC
2630 GGCGCC
NarI 191 GGCGCC GG^CGCC
2630 GGCGCC
PflMI 273 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1075 CCAGGGTGTGG
PmaCI 2111 CACGTG CAC^GTG
3018 CACGTG
PmlI 2111 CACGTG CAC^GTG
3018 CACGTG
PshAI 250 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3518 GACTGCGGTC
PvuII 2938 CAGCTG CAG^CTG
4409 CAGCTG
SfoI 191 GGCGCC GGC^GCC
2630 GGCGCC
SphI 2215 GCATGC GCATG^C
4376 GCATGC
Van91I 273 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1075 CCAGGGTGTGG
XmnI 1908 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3107 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwNI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XmaCI
XmaI