##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbeI BcgI BfrI BglII
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BstBI ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HaeII HindIII HpaI
KasI KpnI Ksp632I MamI MluI
MroI NarI NdeI NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI ScaI SfoI
SfuI SmaI SnaBI SpeI SspBI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 4185 GAATTC [GA]^AATT[CT]
AcyI 1365 GGCGTC G[GA]^CG[CT]C
Alw44I 3004 GTGCAC G^TGCAC
ApaLI 3004 GTGCAC G^TGCAC
ApoI 4185 GAATTC [GA]^AATT[CT]
Asp700I 2568 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3276 CCTAGG C^CTAGG
BbrPI 241 CACGTG CAC^GTG
BbvCI 4026 GCTGAGG CCTCAGC(-5/-2)
BclI 3239 TGATCA T^GATCA
BlnI 3276 CCTAGG C^CTAGG
BsaAI 241 CACGTG [CT]AC^GT[GA]
BsaHI 1365 GGCGTC G[GA]^CG[CT]C
BsrBI 1190 GAGCGG CCGCTC(-3/-3)
Bst1107I 3699 GTATAC GTA^TAC
BstAPI 3206 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3862 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1284 GGATCT [GA]^GATC[CT]
BstZ17I 3699 GTATAC GTA^TAC
EcoRI 4185 GAATTC G^AATTC
FbaI 3239 TGATCA T^GATCA
HgaI 1366 GCGTC GACGC(5/10)
Hin1I 1365 GGCGTC G[GA]^CG[CT]C
KspI 1100 CCGCGG CCGC^GG
MfeI 1558 CAATTG C^AATTG
MflI 1284 GGATCT [GA]^GATC[CT]
MslI 3231 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1558 CAATTG C^AATTG
NruI 2933 TCGCGA TCG^CGA
PaeR7I 2753 CTCGAG C^TCGAG
PflMI 3760 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 241 CACGTG CAC^GTG
PmlI 241 CACGTG CAC^GTG
PshAI 2592 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3776 GAGCTC GAGCT^C
SacII 1100 CCGCGG CCGC^GG
SbfI 504 CCTGCAGG CCTGCA^GG
SgrAI 67 CGCCGGTG C[GA]^CCGG[CT]G
SphI 38 GCATGC GCATG^C
Sse8387I 504 CCTGCAGG CCTGCA^GG
SspI 2979 AATATT AAT^ATT
TfiI 1600 GATTC G^A[AT]TC
Van91I 3760 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3585 TCTAGA T^CTAGA
XhoI 2753 CTCGAG C^TCGAG
XhoII 1284 GGATCT [GA]^GATC[CT]
XmnI 2568 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2770 GTCTAC GT^[AC][GT]AC
3699 GTATAC
AflIII 791 ACGTGT A^C[GA][CT]GT
3794 ACACGT
AlwI 1629 GATCC GGATC(4/5)
1284 GGATC
BalI 27 TGGCCA TGG^CCA
3901 TGGCCA
BciVI 137 GGATAC GTATCC(6/5)
3730 GTATCC
BlpI 125 GCTCAGC GC^T[ACGT]AGC
3490 GCTCAGC
Bpu1102I 125 GCTCAGC GC^T[ACGT]AGC
3490 GCTCAGC
BsgI 430 GTGCAG GTGCAG(16/14)
2626 GTGCAG
BsmI 3130 GCATTC GAATGC(1/-1)
3355 GCATTC
BsrDI 2816 CATTGC GCAATG(2/0)
3947 CATTGC
CelII 125 GCTCAGC GC^T[ACGT]AGC
3490 GCTCAGC
EcoNI 3666 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3977 CCTGAAAAAGG
MluNI 27 TGGCCA TGG^CCA
3901 TGGCCA
MscI 27 TGGCCA TGG^CCA
3901 TGGCCA
MseI 405 TTAA T^TAA
3186 TTAA
NaeI 1290 GCCGGC GCC^GGC
2820 GCCGGC
NcoI 1847 CCATGG C^CATGG
3233 CCATGG
NgoMIV 1290 GCCGGC G^CCGGC
2820 GCCGGC
PstI 505 CTGCAG CTGCA^G
2998 CTGCAG
StuI 986 AGGCCT AGG^CCT
1548 AGGCCT
Tru9I 405 TTAA T^TAA
3186 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfiI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI