##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI BcgI BciVI BfrI
BsaBI BsaI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI Eam1105I
Eco47III EcoO65I EcoT22I FseI HpaI
MamI MfeI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfuI SnaBI Sse8387I SspBI
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 617 GGTACC G^GTACC
AccI 4401 GTCTAC GT^[AC][GT]AC
AcsI 1207 GAATTC [GA]^AATT[CT]
Alw44I 2558 GTGCAC G^TGCAC
ApaLI 2558 GTGCAC G^TGCAC
ApoI 1207 GAATTC [GA]^AATT[CT]
Asp718I 617 GGTACC G^GTACC
AvrII 3031 CCTAGG C^CTAGG
BalI 984 TGGCCA TGG^CCA
BamHI 2023 GGATCC G^GATCC
BclI 605 TGATCA T^GATCA
BglII 423 AGATCT A^GATCT
BlnI 3031 CCTAGG C^CTAGG
BsmI 3896 GAATGC GAATGC(1/-1)
DraIII 2561 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1946 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1207 GAATTC G^AATTC
EcoRV 568 GATATC GAT^ATC
FbaI 605 TGATCA T^GATCA
KpnI 617 GGTACC GGTAC^C
MluI 4038 ACGCGT A^CGCGT
MluNI 984 TGGCCA TGG^CCA
MscI 984 TGGCCA TGG^CCA
NspI 1643 GCATGC [GA]CATG^[CT]
PmeI 1326 GTTTAAAC GTTT^AAAC
PvuII 3384 CAGCTG CAG^CTG
ScaI 261 AGTACT AGT^ACT
SfiI 2611 GGCCACGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3138 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1906 ACTAGT A^CTAGT
SphI 1643 GCATGC GCATG^C
Tsp509I 1208 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 2024 GATCC GGATC(4/5)
2023 GGATC
AocI 944 CCTAAGG CC^T[ACGT]AGG
2251 CCTGAGG
AviII 2495 TGCGCA TGC^GCA
3899 TGCGCA
BlpI 639 GCTCAGC GC^T[ACGT]AGC
2392 GCTGAGC
Bpu1102I 639 GCTCAGC GC^T[ACGT]AGC
2392 GCTGAGC
BsmBI 2795 CGTCTC CGTCTC(1/5)
3134 GAGACG
BsrDI 905 CATTGC GCAATG(2/0)
1505 CATTGC
BstXI 1007 CCAGAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1348 CCACCCCTCTGG
BstYI 423 AGATCT [GA]^GATC[CT]
2023 GGATCC
Bsu36I 944 CCTAAGG CC^T[ACGT]AGG
2251 CCTGAGG
CelII 639 GCTCAGC GC^T[ACGT]AGC
2392 GCTGAGC
DraI 1309 TTTAAA TTT^AAA
1327 TTTAAA
DrdI 3298 GACCCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4250 GACCCCAGCGTC
EagI 272 CGGCCG C^GGCCG
4344 CGGCCG
EclXI 272 CGGCCG C^GGCCG
4344 CGGCCG
Eco52I 272 CGGCCG C^GGCCG
4344 CGGCCG
Eco81I 944 CCTAAGG CC^T[ACGT]AGG
2251 CCTGAGG
FspI 2495 TGCGCA TGC^GCA
3899 TGCGCA
HindIII 1119 AAGCTT A^AGCTT
2004 AAGCTT
MflI 423 AGATCT [GA]^GATC[CT]
2023 GGATCC
NaeI 3139 GCCGGC GCC^GGC
4165 GCCGGC
NgoMIV 3139 GCCGGC G^CCGGC
4165 GCCGGC
PaeR7I 2207 CTCGAG C^TCGAG
3704 CTCGAG
PstI 3127 CTGCAG CTGCA^G
4170 CTGCAG
SmlI 2207 CTCGAG C^T[CT][GA]AG
3704 CTCGAG
StuI 414 AGGCCT AGG^CCT
1939 AGGCCT
TfiI 950 GATTC G^A[AT]TC
4068 GATTC
XhoI 2207 CTCGAG C^TCGAG
3704 CTCGAG
XhoII 423 AGATCT [GA]^GATC[CT]
2023 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI AspHI AspI
AvaI AvaII BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflFI
PflMI PleI PmaCI PmlI PpuMI
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI Tru9I TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XmaCI XmaI