##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI ApaI AscI
Asp718I AspI AvaI AviII BalI
BbeI BbrPI BbvCI BcgI BfrI
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraIII EagI EclXI Eco52I EcoO65I
EcoRI EcoRV FseI FspI Hin1I
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1877 GTCTAC GT^[AC][GT]AC
AhdI 3041 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 677 CCTCAGG CC^T[ACGT]AGG
Aor51HI 417 AGCGCT AGC^GCT
AseI 4356 ATTAAT AT^TAAT
AsnI 4356 ATTAAT AT^TAAT
AspEI 3041 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3014 CCTAGG C^CTAGG
BamHI 4006 GGATCC G^GATCC
BclI 1645 TGATCA T^GATCA
BglI 224 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2765 AGATCT A^GATCT
BlnI 3014 CCTAGG C^CTAGG
BlpI 2191 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2191 GCTGAGC GC^T[ACGT]AGC
BsaBI 2942 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 2816 GCAGGT ACCTGC(4/8)
BsrFI 411 GCCGGC [GA]^CCGG[CT]
Bsu36I 677 CCTCAGG CC^T[ACGT]AGG
CelII 2191 GCTGAGC GC^T[ACGT]AGC
Cfr10I 411 GCCGGC [GA]^CCGG[CT]
DrdI 1938 GACTGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1922 CGGCCA [CT]^GGCC[GA]
Eam1105I 3041 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 417 AGCGCT AGC^GCT
Eco81I 677 CCTCAGG CC^T[ACGT]AGG
FbaI 1645 TGATCA T^GATCA
HaeII 417 AGCGCT [GA]GCGC^[CT]
HgaI 1945 GCGTC GACGC(5/10)
HindIII 530 AAGCTT A^AGCTT
HpaI 214 GTTAAC GTT^AAC
MamI 2942 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 411 GCCGGC GCC^GGC
NcoI 2121 CCATGG C^CATGG
NgoMIV 411 GCCGGC G^CCGGC
PshBI 4356 ATTAAT AT^TAAT
ScaI 708 AGTACT AGT^ACT
StuI 1102 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 93 GTGCAC G^TGCAC
2248 GTGCAC
ApaLI 93 GTGCAC G^TGCAC
2248 GTGCAC
Asp700I 174 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
866 GAAAAACTTC
BanII 284 GAGCTC G[GA]GC[CT]^C
1202 GAGCTC
BcnI 1678 CCCGG CC^[GC]GG
1857 CCCGG
BsaWI 1119 TCCGGT [AT]^CCGG[AT]
1835 ACCGGA
BspHI 117 TCATGA T^CATGA
3778 TCATGA
BspLU11I 2241 ACATGT A^CATGT
4151 ACATGT
BsrDI 34 CATTGC GCAATG(2/0)
646 CATTGC
DsaI 2000 CCACGG C^C[GA][CT]GG
2121 CCATGG
EcoNI 1393 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1534 CCTGGAAGAGG
EcoT22I 3871 ATGCAT ATGCA^T
4346 ATGCAT
FauI 4185 CCCGC CCCGC(4/6)
1468 GCGGG
HincII 214 GTTAAC GT[CT]^[GA]AC
1947 GTCAAC
HindII 214 GTTAAC GT[CT]^[GA]AC
1947 GTCAAC
MaeII 1875 ACGT A^CGT
3846 ACGT
MfeI 2994 CAATTG C^AATTG
3821 CAATTG
MunI 2994 CAATTG C^AATTG
3821 CAATTG
NciI 1678 CCCGG CC^[GC]GG
1857 CCCGG
NsiI 3871 ATGCAT ATGCA^T
4346 ATGCAT
PpuMI 2074 AGGACCC [GA]G^G[AT]CC[CT]
2678 AGGTCCC
RcaI 117 TCATGA T^CATGA
3778 TCATGA
SacI 284 GAGCTC GAGCT^C
1202 GAGCTC
SspI 3259 AATATT AAT^ATT
4450 AATATT
XbaI 3343 TCTAGA T^CTAGA
4042 TCTAGA
XmnI 174 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
866 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII