##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BssHII BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I HaeII Hin1I HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NarI NciI NcoI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I TaqI TthHB8I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2022 GGTACC G^GTACC
AclI 2459 AACGTT AA^CGTT
ApaI 182 GGGCCC GGGCC^C
Asp718I 2022 GGTACC G^GTACC
AspI 3961 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 579 CTCGGG C^[CT]CG[GA]G
AviII 2418 TGCGCA TGC^GCA
BanI 2022 GGTACC G^G[CT][GA]CC
BclI 4016 TGATCA T^GATCA
BglI 3236 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 4359 GGTCTC GGTCTC(1/5)
BsgI 758 GTGCAG GTGCAG(16/14)
BsmBI 3786 GAGACG CGTCTC(1/5)
BsoBI 579 CTCGGG C^[CT]CG[GA]G
BspMI 4626 GCAGGT ACCTGC(4/8)
BsrBI 1751 GAGCGG CCGCTC(-3/-3)
BsrFI 2303 GCCGGC [GA]^CCGG[CT]
BssSI 511 CTCGTG CACGAG(-5/-1)
BstAPI 886 GCAGTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2419 GCGC GCG^C
Cfr10I 2303 GCCGGC [GA]^CCGG[CT]
DraIII 3024 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1580 CCGTGG C^C[GA][CT]GG
EcoRI 1324 GAATTC G^AATTC
EcoRV 2923 GATATC GAT^ATC
EcoT22I 1547 ATGCAT ATGCA^T
FauI 1245 GCGGG CCCGC(4/6)
FbaI 4016 TGATCA T^GATCA
FseI 2302 GGCCGGCC GGCCGG^CC
FspI 2418 TGCGCA TGC^GCA
HapII 2304 CCGG C^CGG
HgaI 949 GACGC GACGC(5/10)
HhaI 2419 GCGC GCG^C
HinP1I 2419 GCGC G^CGC
HindIII 442 AAGCTT A^AGCTT
HpaII 2304 CCGG C^CGG
KpnI 2022 GGTACC GGTAC^C
MspI 2304 CCGG C^CGG
NaeI 2303 GCCGGC GCC^GGC
NgoMIV 2303 GCCGGC G^CCGGC
NsiI 1547 ATGCAT ATGCA^T
PflFI 3961 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3023 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1760 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2459 AACGTT AA^CGTT
ScaI 4452 AGTACT AGT^ACT
SphI 1779 GCATGC GCATG^C
SwaI 3725 ATTTAAAT ATTT^AAAT
Tth111I 3961 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3023 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3931 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 796 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4054 GAAAGATTTC
BglII 504 AGATCT A^GATCT
1029 AGATCT
BseRI 356 CTCCTC GAGGAG(10/8)
350 GAGGAG
BsiYI 23 CCCACTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3023 CCACTGGGTGG
BslI 23 CCCACTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3023 CCACTGGGTGG
BsmFI 1247 GGGAC GGGAC(10/14)
4074 GTCCC
Bsp1407I 563 TGTACA T^GTACA
3264 TGTACA
BsrGI 563 TGTACA T^GTACA
3264 TGTACA
Bst1107I 3767 GTATAC GTA^TAC
4214 GTATAC
BstZ17I 3767 GTATAC GTA^TAC
4214 GTATAC
EcoT14I 1287 CCTTGG C^C[AT][AT]GG
3632 CCTTGG
HincII 774 GTCAAC GT[CT]^[GA]AC
946 GTTGAC
HindII 774 GTCAAC GT[CT]^[GA]AC
946 GTTGAC
MslI 1182 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2469 CACCATAGTG
PstI 1671 CTGCAG CTGCA^G
4127 CTGCAG
SmlI 1704 CTCAAG C^T[CT][GA]AG
2341 CTCAAG
SspBI 563 TGTACA T^GTACA
3264 TGTACA
StuI 4123 AGGCCT AGG^CCT
4473 AGGCCT
StyI 1287 CCTTGG C^C[AT][AT]GG
3632 CCTTGG
XmnI 796 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4054 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsmAI
BsmI Bsp1286I BspHI BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII