##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbeI
BcgI BclI BlnI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EcoO65I EcoT22I FbaI
FseI FspI HpaI KasI KpnI
MamI MfeI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SfoI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2740 GTCTAC GT^[AC][GT]AC
AcsI 3205 GAATTC [GA]^AATT[CT]
AfaI 584 GTAC GT^AC
AflII 4274 CTTAAG C^TTAAG
Aor51HI 760 AGCGCT AGC^GCT
ApoI 3205 GAATTC [GA]^AATT[CT]
BciVI 2960 GTATCC GTATCC(6/5)
BfrI 4274 CTTAAG C^TTAAG
BglII 3007 AGATCT A^GATCT
BspHI 793 TCATGA T^CATGA
BsrBI 1051 CCGCTC CCGCTC(-3/-3)
BsrDI 3418 CATTGC GCAATG(2/0)
BssHII 2360 GCGCGC G^CGCGC
Eco47III 760 AGCGCT AGC^GCT
EcoRI 3205 GAATTC G^AATTC
EcoRV 1644 GATATC GAT^ATC
HincII 2034 GTTGAC GT[CT]^[GA]AC
HindII 2034 GTTGAC GT[CT]^[GA]AC
HindIII 2716 AAGCTT A^AGCTT
KspI 4032 CCGCGG CCGC^GG
MluI 4439 ACGCGT A^CGCGT
NspI 2672 GCATGT [GA]CATG^[CT]
PshAI 1373 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 793 TCATGA T^CATGA
RsaI 584 GTAC GT^AC
SacI 1993 GAGCTC GAGCT^C
SacII 4032 CCGCGG CCGC^GG
SexAI 4179 ACCAGGT A^CC[AT]GGT
SgrAI 1671 CACCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 789 GGCGTC G[GA]^CG[CT]C
3507 GACGCC
AlwI 2003 GATCC GGATC(4/5)
1694 GGATC
BbsI 1503 GAAGAC GAAGAC(2/6)
555 GTCTTC
BlpI 1397 GCTCAGC GC^T[ACGT]AGC
1926 GCTCAGC
Bpu1102I 1397 GCTCAGC GC^T[ACGT]AGC
1926 GCTCAGC
BpuAI 1503 GAAGAC GAAGAC(2/6)
555 GTCTTC
BsaHI 789 GGCGTC G[GA]^CG[CT]C
3507 GACGCC
BsiEI 1714 CGGCCG CG[GA][CT]^CG
2230 CGGCCG
BssSI 516 CACGAG CACGAG(-5/-1)
1035 CACGAG
BstYI 1694 GGATCT [GA]^GATC[CT]
3007 AGATCT
CelII 1397 GCTCAGC GC^T[ACGT]AGC
1926 GCTCAGC
DraI 1961 TTTAAA TTT^AAA
4096 TTTAAA
EagI 1714 CGGCCG C^GGCCG
2230 CGGCCG
EclXI 1714 CGGCCG C^GGCCG
2230 CGGCCG
Eco52I 1714 CGGCCG C^GGCCG
2230 CGGCCG
HaeII 760 AGCGCT [GA]GCGC^[CT]
3079 GGCGCT
Hin1I 789 GGCGTC G[GA]^CG[CT]C
3507 GACGCC
MflI 1694 GGATCT [GA]^GATC[CT]
3007 AGATCT
NaeI 281 GCCGGC GCC^GGC
606 GCCGGC
NcoI 468 CCATGG C^CATGG
896 CCATGG
NgoMIV 281 GCCGGC G^CCGGC
606 GCCGGC
SbfI 746 CCTGCAGG CCTGCA^GG
3385 CCTGCAGG
SfiI 3482 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3947 GGCCAGCCTGGCC
Sse8387I 746 CCTGCAGG CCTGCA^GG
3385 CCTGCAGG
TaqI 1096 TCGA T^CGA
2457 TCGA
Tsp509I 3206 AATT ^AATT
4106 AATT
TthHB8I 1096 TCGA T^CGA
2457 TCGA
XcmI 3493 CCACAGCTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4406 CCACGCCCAGGCTGG
XhoII 1694 GGATCT [GA]^GATC[CT]
3007 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AflIII AhdI AluI
Alw44I AlwNI AocI ApaI ApaLI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I TspRI Van91I XmaCI XmaI