##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI Asp700I Asp718I AspEI
AviII BamHI BbeI BbrPI BciVI
BsaBI BsaHI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraIII DrdI Eam1105I EcoRV EcoT22I
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI NarI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1334 GTAGAC GT^[AC][GT]AC
AccII 1375 CGCG CG^CG
AflII 749 CTTAAG C^TTAAG
AocI 2187 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1402 AGCGCT AGC^GCT
AseI 1251 ATTAAT AT^TAAT
AsnI 1251 ATTAAT AT^TAAT
AspI 3855 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3542 CCTAGG C^CTAGG
BcgI 2825 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 749 CTTAAG C^TTAAG
BlnI 3542 CCTAGG C^CTAGG
BlpI 1316 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1316 GCTGAGC GC^T[ACGT]AGC
BsaAI 591 TACGTG [CT]AC^GT[GA]
BsaI 2937 GGTCTC GGTCTC(1/5)
BsiEI 2037 CGGCCG CG[GA][CT]^CG
BsmBI 1930 GAGACG CGTCTC(1/5)
BsrFI 2039 GCCGGC [GA]^CCGG[CT]
BstUI 1375 CGCG CG^CG
Bsu36I 2187 CCTGAGG CC^T[ACGT]AGG
CelII 1316 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2039 GCCGGC [GA]^CCGG[CT]
DraII 2246 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 2037 CGGCCG C^GGCCG
EclXI 2037 CGGCCG C^GGCCG
Eco47III 1402 AGCGCT AGC^GCT
Eco52I 2037 CGGCCG C^GGCCG
Eco81I 2187 CCTGAGG CC^T[ACGT]AGG
EcoNI 3537 CCTTCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2246 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 3569 GAATTC G^AATTC
FseI 2038 GGCCGGCC GGCCGG^CC
HindIII 887 AAGCTT A^AGCTT
MfeI 3461 CAATTG C^AATTG
MunI 3461 CAATTG C^AATTG
MvnI 1375 CGCG CG^CG
NaeI 2039 GCCGGC GCC^GGC
NgoMIV 2039 GCCGGC G^CCGGC
NheI 3404 GCTAGC G^CTAGC
PflFI 3855 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2246 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1251 ATTAAT AT^TAAT
SacI 3677 GAGCTC GAGCT^C
StuI 3760 AGGCCT AGG^CCT
Tth111I 3855 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 2421 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3178 CAGTCACTG
AspHI 948 GAGCAC G[AT]GC[AT]^C
3677 GAGCTC
AvaII 2247 GGACC G^G[AT]CC
3107 GGACC
BanI 2865 GGCACC G^G[CT][GA]CC
3130 GGCACC
BclI 962 TGATCA T^GATCA
2317 TGATCA
BglI 1943 GCCGTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2030 GCCACCACGGC
BglII 1013 AGATCT A^GATCT
3225 AGATCT
BsiHKAI 948 GAGCAC G[AT]GC[AT]^C
3677 GAGCTC
BsmI 3047 GAATGC GAATGC(1/-1)
3204 GCATTC
BspHI 1276 TCATGA T^CATGA
3194 TCATGA
BstEII 1092 GGTTACC G^GT[ACGT]ACC
1565 GGTCACC
BstPI 1092 GGTTACC G^GT[ACGT]ACC
1565 GGTCACC
DraI 3765 TTTAAA TTT^AAA
3905 TTTAAA
DsaI 1058 CCGTGG C^C[GA][CT]GG
2034 CCACGG
EarI 943 GAAGAG CTCTTC(1/4)
3101 GAAGAG
EcoO65I 1092 GGTTACC G^GT[ACGT]ACC
1565 GGTCACC
FauI 2869 CCCGC CCCGC(4/6)
1376 GCGGG
FbaI 962 TGATCA T^GATCA
2317 TGATCA
HaeII 1402 AGCGCT [GA]GCGC^[CT]
3698 AGCGCC
Ksp632I 943 GAAGAG CTCTTC(1/4)
3101 GAAGAG
PflMI 3332 CCATATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3501 CCAGTCAATGG
RcaI 1276 TCATGA T^CATGA
3194 TCATGA
SmaI 1836 CCCGGG CCC^GGG
2813 CCCGGG
SpeI 1045 ACTAGT A^CTAGT
3792 ACTAGT
SphI 1523 GCATGC GCATG^C
3654 GCATGC
TaqI 1585 TCGA T^CGA
3349 TCGA
TthHB8I 1585 TCGA T^CGA
3349 TCGA
Van91I 3332 CCATATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3501 CCAGTCAATGG
XbaI 2144 TCTAGA T^CTAGA
2374 TCTAGA
XmaCI 1836 CCCGGG C^CCGGG
2813 CCCGGG
XmaI 1836 CCCGGG C^CCGGG
2813 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BalI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII