##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AclI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI BbeI BsaI BsiEI
BsiWI BsmBI BspHI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3131 GACGTC GACGT^C
AccIII 663 TCCGGA T^CCGGA
AcsI 3759 AAATTT [GA]^AATT[CT]
AflII 3772 CTTAAG C^TTAAG
AgeI 1956 ACCGGT A^CCGGT
AocI 296 CCTGAGG CC^T[ACGT]AGG
ApoI 3759 AAATTT [GA]^AATT[CT]
AviII 248 TGCGCA TGC^GCA
AvrII 1538 CCTAGG C^CTAGG
BamHI 694 GGATCC G^GATCC
BbrPI 980 CACGTG CAC^GTG
BcgI 2513 GCAGGGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1189 GTATCC GTATCC(6/5)
BclI 2820 TGATCA T^GATCA
BfrI 3772 CTTAAG C^TTAAG
BlnI 1538 CCTAGG C^CTAGG
BsaAI 980 CACGTG [CT]AC^GT[GA]
BsaBI 493 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 663 TCCGGA T^CCGGA
BsmI 3052 GCATTC GAATGC(1/-1)
Bsp1407I 3414 TGTACA T^GTACA
BspDI 2898 ATCGAT AT^CGAT
BspEI 663 TCCGGA T^CCGGA
BsrGI 3414 TGTACA T^GTACA
BstAPI 2619 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 296 CCTGAGG CC^T[ACGT]AGG
ClaI 2898 ATCGAT AT^CGAT
Eco81I 296 CCTGAGG CC^T[ACGT]AGG
FbaI 2820 TGATCA T^GATCA
FspI 248 TGCGCA TGC^GCA
HincII 2633 GTCAAC GT[CT]^[GA]AC
HindII 2633 GTCAAC GT[CT]^[GA]AC
HindIII 3419 AAGCTT A^AGCTT
MamI 493 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 663 TCCGGA T^CCGGA
NdeI 3766 CATATG CA^TATG
NheI 1276 GCTAGC G^CTAGC
PflMI 1365 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1956 ACCGGT A^CCGGT
PmaCI 980 CACGTG CAC^GTG
PmlI 980 CACGTG CAC^GTG
SapI 3825 GAAGAGC GCTCTTC(1/4)
SmlI 3772 CTTAAG C^T[CT][GA]AG
SspBI 3414 TGTACA T^GTACA
Van91I 1365 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2775 CCACCTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspI 497 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3332 GACGAGGTC
BbvCI 2392 CCTCAGC CCTCAGC(-5/-2)
2917 CCTCAGC
BglI 1664 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1853 GCCGTGGGGGC
BsaWI 663 TCCGGA [AT]^CCGG[AT]
1956 ACCGGT
BseRI 116 GAGGAG GAGGAG(10/8)
3155 GAGGAG
BspLU11I 309 ACATGT A^CATGT
1221 ACATGT
BspMI 2283 ACCTGC ACCTGC(4/8)
2468 GCAGGT
BstXI 1977 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3354 CCATCATCATGG
DrdI 1997 GACGCCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2378 GACCTGGGTGTC
FauI 280 CCCGC CCCGC(4/6)
1320 CCCGC
PflFI 497 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3332 GACGAGGTC
PshAI 2084 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2529 GACGGGTGTC
ScaI 621 AGTACT AGT^ACT
2847 AGTACT
SexAI 1302 ACCTGGT A^CC[AT]GGT
3647 ACCAGGT
SfiI 1663 GGCCATTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1852 GGCCGTGGGGGCC
SmaI 1675 CCCGGG CCC^GGG
1863 CCCGGG
StuI 715 AGGCCT AGG^CCT
2404 AGGCCT
Tth111I 497 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3332 GACGAGGTC
XmaCI 1675 CCCGGG C^CCGGG
1863 CCCGGG
XmaI 1675 CCCGGG C^CCGGG
1863 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII