##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Aor51HI ApaI AscI AspEI
AviII BbeI BbrPI BclI BfrI
BglI BlpI Bpu1102I BsaBI BsaHI
BsiEI BsiWI Bsp1407I BspDI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FbaI FseI FspI HaeII
HgaI Hin1I KasI KspI MamI
MluI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SpeI Sse8387I SspBI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 4749 GGTACC G^GTACC
AccII 67 CGCG CG^CG
AccIII 200 TCCGGA T^CCGGA
Alw44I 2815 GTGCAC G^TGCAC
ApaLI 2815 GTGCAC G^TGCAC
AseI 3935 ATTAAT AT^TAAT
AsnI 3935 ATTAAT AT^TAAT
Asp700I 3826 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4749 GGTACC G^GTACC
AvrII 4591 CCTAGG C^CTAGG
BalI 2320 TGGCCA TGG^CCA
BamHI 3781 GGATCC G^GATCC
BbvCI 207 CCTCAGC CCTCAGC(-5/-2)
BcgI 223 CGATTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1778 GGATAC GTATCC(6/5)
BlnI 4591 CCTAGG C^CTAGG
BsaI 116 GAGACC GGTCTC(1/5)
BsaWI 200 TCCGGA [AT]^CCGG[AT]
BseAI 200 TCCGGA T^CCGGA
BseRI 3986 CTCCTC GAGGAG(10/8)
BsgI 2857 GTGCAG GTGCAG(16/14)
BsmBI 2597 GAGACG CGTCTC(1/5)
BspEI 200 TCCGGA T^CCGGA
BspLU11I 2777 ACATGT A^CATGT
BstUI 67 CGCG CG^CG
CfoI 68 GCGC GCG^C
DraII 1006 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2320 TGGCCA [CT]^GGCC[GA]
EcoO109I 1006 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1032 GAATTC G^AATTC
EcoT22I 290 ATGCAT ATGCA^T
FauI 758 GCGGG CCCGC(4/6)
HhaI 68 GCGC GCG^C
HinP1I 68 GCGC G^CGC
HindIII 4795 AAGCTT A^AGCTT
HpaI 1185 GTTAAC GTT^AAC
KpnI 4749 GGTACC GGTAC^C
MluNI 2320 TGGCCA TGG^CCA
MroI 200 TCCGGA T^CCGGA
MscI 2320 TGGCCA TGG^CCA
MvnI 67 CGCG CG^CG
NdeI 3661 CATATG CA^TATG
NsiI 290 ATGCAT ATGCA^T
PaeR7I 3273 CTCGAG C^TCGAG
PshAI 3138 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3935 ATTAAT AT^TAAT
SapI 167 GAAGAGC GCTCTTC(1/4)
SmaI 3677 CCCGGG CCC^GGG
SnaBI 4401 TACGTA TAC^GTA
SphI 2023 GCATGC GCATG^C
StuI 2784 AGGCCT AGG^CCT
SwaI 3120 ATTTAAAT ATTT^AAAT
XcmI 4302 CCATGTTCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3273 CTCGAG C^TCGAG
XmaCI 3677 CCCGGG C^CCGGG
XmaI 3677 CCCGGG C^CCGGG
XmnI 3826 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3846 GTATAC GT^[AC][GT]AC
4882 GTATAC
AflIII 2777 ACATGT A^C[GA][CT]GT
3820 ACGTGT
AspHI 705 GAGCAC G[AT]GC[AT]^C
2815 GTGCAC
AspI 2568 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4858 GACTATGTC
AvaI 3273 CTCGAG C^[CT]CG[GA]G
3677 CCCGGG
BanI 2270 GGTGCC G^G[CT][GA]CC
4749 GGTACC
BanII 132 GAGCCC G[GA]GC[CT]^C
3154 GGGCTC
BglII 1307 AGATCT A^GATCT
1385 AGATCT
BsaAI 3819 TACGTG [CT]AC^GT[GA]
4401 TACGTA
BsiHKAI 705 GAGCAC G[AT]GC[AT]^C
2815 GTGCAC
BsoBI 3273 CTCGAG C^[CT]CG[GA]G
3677 CCCGGG
BspHI 3506 TCATGA T^CATGA
3689 TCATGA
BspMI 413 GCAGGT ACCTGC(4/8)
2516 GCAGGT
Bst1107I 3846 GTATAC GTA^TAC
4882 GTATAC
BstAPI 2018 GCAGGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2719 GCATTTCTTGC
BstEII 3636 GGTCACC G^GT[ACGT]ACC
3682 GGTGACC
BstPI 3636 GGTCACC G^GT[ACGT]ACC
3682 GGTGACC
BstZ17I 3846 GTATAC GTA^TAC
4882 GTATAC
DsaI 94 CCATGG C^C[GA][CT]GG
2810 CCATGG
EcoNI 2483 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3196 CCTTACAGAGG
EcoO65I 3636 GGTCACC G^GT[ACGT]ACC
3682 GGTGACC
EcoRV 1641 GATATC GAT^ATC
4061 GATATC
HincII 1185 GTTAAC GT[CT]^[GA]AC
1690 GTCAAC
HindII 1185 GTTAAC GT[CT]^[GA]AC
1690 GTCAAC
MfeI 448 CAATTG C^AATTG
2666 CAATTG
MunI 448 CAATTG C^AATTG
2666 CAATTG
NcoI 94 CCATGG C^CATGG
2810 CCATGG
PflFI 2568 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4858 GACTATGTC
PflMI 938 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3884 CCACATTTTGG
PvuII 2731 CAGCTG CAG^CTG
4684 CAGCTG
RcaI 3506 TCATGA T^CATGA
3689 TCATGA
SexAI 830 ACCAGGT A^CC[AT]GGT
4074 ACCAGGT
Tth111I 2568 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4858 GACTATGTC
Van91I 938 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3884 CCACATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI EarI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII