################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AspEI AviII BbeI BbrPI BclI BfrI BglI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI BstBI CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I FbaI FseI FspI HaeII HgaI Hin1I KasI KspI MamI MluI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspBI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 4749 GGTACC G^GTACC AccII 67 CGCG CG^CG AccIII 200 TCCGGA T^CCGGA Alw44I 2815 GTGCAC G^TGCAC ApaLI 2815 GTGCAC G^TGCAC AseI 3935 ATTAAT AT^TAAT AsnI 3935 ATTAAT AT^TAAT Asp700I 3826 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4749 GGTACC G^GTACC AvrII 4591 CCTAGG C^CTAGG BalI 2320 TGGCCA TGG^CCA BamHI 3781 GGATCC G^GATCC BbvCI 207 CCTCAGC CCTCAGC(-5/-2) BcgI 223 CGATTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1778 GGATAC GTATCC(6/5) BlnI 4591 CCTAGG C^CTAGG BsaI 116 GAGACC GGTCTC(1/5) BsaWI 200 TCCGGA [AT]^CCGG[AT] BseAI 200 TCCGGA T^CCGGA BseRI 3986 CTCCTC GAGGAG(10/8) BsgI 2857 GTGCAG GTGCAG(16/14) BsmBI 2597 GAGACG CGTCTC(1/5) BspEI 200 TCCGGA T^CCGGA BspLU11I 2777 ACATGT A^CATGT BstUI 67 CGCG CG^CG CfoI 68 GCGC GCG^C DraII 1006 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 2320 TGGCCA [CT]^GGCC[GA] EcoO109I 1006 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 1032 GAATTC G^AATTC EcoT22I 290 ATGCAT ATGCA^T FauI 758 GCGGG CCCGC(4/6) HhaI 68 GCGC GCG^C HinP1I 68 GCGC G^CGC HindIII 4795 AAGCTT A^AGCTT HpaI 1185 GTTAAC GTT^AAC KpnI 4749 GGTACC GGTAC^C MluNI 2320 TGGCCA TGG^CCA MroI 200 TCCGGA T^CCGGA MscI 2320 TGGCCA TGG^CCA MvnI 67 CGCG CG^CG NdeI 3661 CATATG CA^TATG NsiI 290 ATGCAT ATGCA^T PaeR7I 3273 CTCGAG C^TCGAG PshAI 3138 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3935 ATTAAT AT^TAAT SapI 167 GAAGAGC GCTCTTC(1/4) SmaI 3677 CCCGGG CCC^GGG SnaBI 4401 TACGTA TAC^GTA SphI 2023 GCATGC GCATG^C StuI 2784 AGGCCT AGG^CCT SwaI 3120 ATTTAAAT ATTT^AAAT XcmI 4302 CCATGTTCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3273 CTCGAG C^TCGAG XmaCI 3677 CCCGGG C^CCGGG XmaI 3677 CCCGGG C^CCGGG XmnI 3826 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 3846 GTATAC GT^[AC][GT]AC 4882 GTATAC AflIII 2777 ACATGT A^C[GA][CT]GT 3820 ACGTGT AspHI 705 GAGCAC G[AT]GC[AT]^C 2815 GTGCAC AspI 2568 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4858 GACTATGTC AvaI 3273 CTCGAG C^[CT]CG[GA]G 3677 CCCGGG BanI 2270 GGTGCC G^G[CT][GA]CC 4749 GGTACC BanII 132 GAGCCC G[GA]GC[CT]^C 3154 GGGCTC BglII 1307 AGATCT A^GATCT 1385 AGATCT BsaAI 3819 TACGTG [CT]AC^GT[GA] 4401 TACGTA BsiHKAI 705 GAGCAC G[AT]GC[AT]^C 2815 GTGCAC BsoBI 3273 CTCGAG C^[CT]CG[GA]G 3677 CCCGGG BspHI 3506 TCATGA T^CATGA 3689 TCATGA BspMI 413 GCAGGT ACCTGC(4/8) 2516 GCAGGT Bst1107I 3846 GTATAC GTA^TAC 4882 GTATAC BstAPI 2018 GCAGGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2719 GCATTTCTTGC BstEII 3636 GGTCACC G^GT[ACGT]ACC 3682 GGTGACC BstPI 3636 GGTCACC G^GT[ACGT]ACC 3682 GGTGACC BstZ17I 3846 GTATAC GTA^TAC 4882 GTATAC DsaI 94 CCATGG C^C[GA][CT]GG 2810 CCATGG EcoNI 2483 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3196 CCTTACAGAGG EcoO65I 3636 GGTCACC G^GT[ACGT]ACC 3682 GGTGACC EcoRV 1641 GATATC GAT^ATC 4061 GATATC HincII 1185 GTTAAC GT[CT]^[GA]AC 1690 GTCAAC HindII 1185 GTTAAC GT[CT]^[GA]AC 1690 GTCAAC MfeI 448 CAATTG C^AATTG 2666 CAATTG MunI 448 CAATTG C^AATTG 2666 CAATTG NcoI 94 CCATGG C^CATGG 2810 CCATGG PflFI 2568 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4858 GACTATGTC PflMI 938 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3884 CCACATTTTGG PvuII 2731 CAGCTG CAG^CTG 4684 CAGCTG RcaI 3506 TCATGA T^CATGA 3689 TCATGA SexAI 830 ACCAGGT A^CC[AT]GGT 4074 ACCAGGT Tth111I 2568 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4858 GACTATGTC Van91I 938 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3884 CCACATTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI EarI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII