##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI BbeI BbsI
BclI BfrI BglI BpuAI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRV FbaI
FseI HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 239 GACGTC GACGT^C
AhdI 2065 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3815 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2065 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3125 TGCGCA TGC^GCA
AvrII 4222 CCTAGG C^CTAGG
BamHI 2673 GGATCC G^GATCC
BlnI 4222 CCTAGG C^CTAGG
BlpI 2961 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2961 GCTCAGC GC^T[ACGT]AGC
BsaBI 342 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1002 CGATCG CG[GA][CT]^CG
Bsp1407I 4059 TGTACA T^GTACA
BspHI 1985 TCATGA T^CATGA
BsrGI 4059 TGTACA T^GTACA
BstEII 2989 GGTCACC G^GT[ACGT]ACC
BstPI 2989 GGTCACC G^GT[ACGT]ACC
CelII 2961 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2065 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2989 GGTCACC G^GT[ACGT]ACC
EcoT22I 2162 ATGCAT ATGCA^T
FspI 3125 TGCGCA TGC^GCA
HincII 925 GTCAAC GT[CT]^[GA]AC
HindII 925 GTCAAC GT[CT]^[GA]AC
MamI 342 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2162 ATGCAT ATGCA^T
PaeR7I 1882 CTCGAG C^TCGAG
PflMI 1291 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3850 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1002 CGATCG CGAT^CG
RcaI 1985 TCATGA T^CATGA
SacI 2731 GAGCTC GAGCT^C
SmaI 4053 CCCGGG CCC^GGG
SpeI 372 ACTAGT A^CTAGT
SspBI 4059 TGTACA T^GTACA
Van91I 1291 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1882 CTCGAG C^TCGAG
XmaCI 4053 CCCGGG C^CCGGG
XmaI 4053 CCCGGG C^CCGGG
XmnI 3815 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1522 GTGCAC G^TGCAC
2626 GTGCAC
AocI 2799 CCTGAGG CC^T[ACGT]AGG
3975 CCTGAGG
ApaLI 1522 GTGCAC G^TGCAC
2626 GTGCAC
BalI 1394 TGGCCA TGG^CCA
4017 TGGCCA
BbrPI 2224 CACGTG CAC^GTG
2956 CACGTG
BcgI 1510 GCAGGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1916 GCATCTCCTTCG
BciVI 438 GTATCC GTATCC(6/5)
1322 GTATCC
BglII 713 AGATCT A^GATCT
1058 AGATCT
BsrDI 2894 GCAATG GCAATG(2/0)
3729 GCAATG
BsrFI 204 GCCGGC [GA]^CCGG[CT]
3160 GCCGGC
Bsu36I 2799 CCTGAGG CC^T[ACGT]AGG
3975 CCTGAGG
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
3160 GCCGGC
DraIII 1289 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2554 CACCAGGTG
DrdI 135 GACGCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
643 GACAACACGGTC
Eco81I 2799 CCTGAGG CC^T[ACGT]AGG
3975 CCTGAGG
EcoRI 2899 GAATTC G^AATTC
3422 GAATTC
HaeII 1431 GGCGCT [GA]GCGC^[CT]
3058 AGCGCC
HindIII 53 AAGCTT A^AGCTT
3816 AAGCTT
MluNI 1394 TGGCCA TGG^CCA
4017 TGGCCA
MscI 1394 TGGCCA TGG^CCA
4017 TGGCCA
NaeI 204 GCCGGC GCC^GGC
3160 GCCGGC
NgoMIV 204 GCCGGC G^CCGGC
3160 GCCGGC
PmaCI 2224 CACGTG CAC^GTG
2956 CACGTG
PmlI 2224 CACGTG CAC^GTG
2956 CACGTG
SmlI 1801 CTTGAG C^T[CT][GA]AG
1882 CTCGAG
StuI 851 AGGCCT AGG^CCT
3393 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII