##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AviII
AvrII BbeI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI MamI MfeI MluI MroI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 664 GGTACC G^GTACC
AflII 592 CTTAAG C^TTAAG
Asp700I 2283 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 664 GGTACC G^GTACC
BamHI 2063 GGATCC G^GATCC
BbsI 3171 GAAGAC GAAGAC(2/6)
BcgI 3836 GCACCAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 15 GTATCC GTATCC(6/5)
BclI 2429 TGATCA T^GATCA
BfrI 592 CTTAAG C^TTAAG
BglII 2109 AGATCT A^GATCT
BpuAI 3171 GAAGAC GAAGAC(2/6)
BsmBI 1520 GAGACG CGTCTC(1/5)
BspHI 263 TCATGA T^CATGA
BssHII 2606 GCGCGC G^CGCGC
BssSI 4127 CACGAG CACGAG(-5/-1)
BstAPI 3579 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4183 TTTAAA TTT^AAA
FbaI 2429 TGATCA T^GATCA
KpnI 664 GGTACC GGTAC^C
RcaI 263 TCATGA T^CATGA
SacI 2932 GAGCTC GAGCT^C
SspI 4218 AATATT AAT^ATT
XbaI 2883 TCTAGA T^CTAGA
XmnI 2283 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 2868 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4021 GACCGAGGGTC
AspEI 2868 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4021 GACCGAGGGTC
BalI 770 TGGCCA TGG^CCA
3149 TGGCCA
BbrPI 774 CACGTG CAC^GTG
3049 CACGTG
BbvCI 2807 CCTCAGC CCTCAGC(-5/-2)
1155 GCTGAGG
BsaAI 774 CACGTG [CT]AC^GT[GA]
3049 CACGTG
BsmI 2689 GCATTC GAATGC(1/-1)
3539 GCATTC
EagI 1236 CGGCCG C^GGCCG
2042 CGGCCG
Eam1105I 2868 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4021 GACCGAGGGTC
EclXI 1236 CGGCCG C^GGCCG
2042 CGGCCG
Eco52I 1236 CGGCCG C^GGCCG
2042 CGGCCG
HgaI 3174 GACGC GACGC(5/10)
2967 GCGTC
MluNI 770 TGGCCA TGG^CCA
3149 TGGCCA
MscI 770 TGGCCA TGG^CCA
3149 TGGCCA
NaeI 546 GCCGGC GCC^GGC
2359 GCCGGC
NcoI 2268 CCATGG C^CATGG
3623 CCATGG
NgoMIV 546 GCCGGC G^CCGGC
2359 GCCGGC
PmaCI 774 CACGTG CAC^GTG
3049 CACGTG
PmlI 774 CACGTG CAC^GTG
3049 CACGTG
SapI 1030 GAAGAGC GCTCTTC(1/4)
3028 GAAGAGC
SbfI 2172 CCTGCAGG CCTGCA^GG
4036 CCTGCAGG
SfiI 2529 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3816 GGCCTTCCAGGCC
Sse8387I 2172 CCTGCAGG CCTGCA^GG
4036 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI