##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AviII BbeI
BciVI BclI BfrI BsaHI BseAI
BsiEI BsiWI BspEI BspHI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
DsaI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KspI
MluI MroI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacII
SapI ScaI SexAI SfoI SfuI
SgrAI SnaBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1261 GGTACC G^GTACC
AccI 1920 GTCGAC GT^[AC][GT]AC
AhdI 2143 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 801 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1261 GGTACC G^GTACC
AspEI 2143 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4065 CCTAGG C^CTAGG
BamHI 3816 GGATCC G^GATCC
BbrPI 2736 CACGTG CAC^GTG
BcgI 1576 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4065 CCTAGG C^CTAGG
BlpI 2685 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2685 GCTCAGC GC^T[ACGT]AGC
BsaAI 2736 CACGTG [CT]AC^GT[GA]
BsaBI 1548 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 321 GAGACC GGTCTC(1/5)
BsaWI 3806 TCCGGT [AT]^CCGG[AT]
BsmBI 2995 GAGACG CGTCTC(1/5)
Bsp1407I 893 TGTACA T^GTACA
BspDI 1645 ATCGAT AT^CGAT
BsrFI 1124 GCCGGC [GA]^CCGG[CT]
BsrGI 893 TGTACA T^GTACA
CelII 2685 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1124 GCCGGC [GA]^CCGG[CT]
ClaI 1645 ATCGAT AT^CGAT
CpoI 1396 CGGACCG CG^G[AT]CCG
DraI 2864 TTTAAA TTT^AAA
Eam1105I 2143 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1284 GATATC GAT^ATC
HincII 1920 GTCGAC GT[CT]^[GA]AC
HindII 1920 GTCGAC GT[CT]^[GA]AC
KpnI 1261 GGTACC GGTAC^C
MamI 1548 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1100 CAATTG C^AATTG
MunI 1100 CAATTG C^AATTG
NaeI 1124 GCCGGC GCC^GGC
NgoMIV 1124 GCCGGC G^CCGGC
PaeR7I 755 CTCGAG C^TCGAG
PmaCI 2736 CACGTG CAC^GTG
PmlI 2736 CACGTG CAC^GTG
RsrII 1396 CGGACCG CG^G[AT]CCG
SacI 1087 GAGCTC GAGCT^C
SalI 1920 GTCGAC G^TCGAC
SbfI 3624 CCTGCAGG CCTGCA^GG
SmaI 1784 CCCGGG CCC^GGG
SpeI 2239 ACTAGT A^CTAGT
Sse8387I 3624 CCTGCAGG CCTGCA^GG
SspBI 893 TGTACA T^GTACA
SspI 506 AATATT AAT^ATT
XhoI 755 CTCGAG C^TCGAG
XmaCI 1784 CCCGGG C^CCGGG
XmaI 1784 CCCGGG C^CCGGG
XmnI 801 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1893 GAATTT [GA]^AATT[CT]
2868 AAATTT
ApoI 1893 GAATTT [GA]^AATT[CT]
2868 AAATTT
BbvCI 3486 CCTCAGC CCTCAGC(-5/-2)
3618 CCTCAGC
BglII 749 AGATCT A^GATCT
953 AGATCT
BsgI 517 GTGCAG GTGCAG(16/14)
1492 GTGCAG
BsmI 3153 GCATTC GAATGC(1/-1)
3429 GCATTC
BspLU11I 1631 ACATGT A^CATGT
4138 ACATGT
BsrBI 3041 CCGCTC CCGCTC(-3/-3)
1423 GAGCGG
BssSI 1608 CACGAG CACGAG(-5/-1)
3938 CACGAG
BstEII 912 GGTGACC G^GT[ACGT]ACC
2931 GGTAACC
BstPI 912 GGTGACC G^GT[ACGT]ACC
2931 GGTAACC
DraIII 120 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3793 CACACTGTG
EcoO65I 912 GGTGACC G^GT[ACGT]ACC
2931 GGTAACC
HindIII 133 AAGCTT A^AGCTT
3465 AAGCTT
SfiI 2711 GGCCATCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2939 GGCCCCTCTGGCC
SphI 2292 GCATGC GCATG^C
2985 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII