################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AclI AflII AflIII AscI AseI AsnI Asp718I AvrII BamHI BbeI BbrPI BfrI BlnI BsaAI BsaBI BsiWI BsmBI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoRV FseI HpaI KasI KpnI MamI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SbfI ScaI SfoI SfuI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 1539 GACGTC GACGT^C AcyI 1539 GACGTC G[GA]^CG[CT]C AhdI 835 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3762 CCTGAGG CC^T[ACGT]AGG Asp700I 1642 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 835 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 1864 CCTCAGC CCTCAGC(-5/-2) BcgI 1534 GCACTGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3622 TGATCA T^GATCA BsaHI 1539 GACGTC G[GA]^CG[CT]C BsiEI 42 CGGCCG CG[GA][CT]^CG Bsu36I 3762 CCTGAGG CC^T[ACGT]AGG DraIII 1205 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 42 CGGCCG C^GGCCG Eam1105I 835 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 42 CGGCCG C^GGCCG Eco52I 42 CGGCCG C^GGCCG Eco81I 3762 CCTGAGG CC^T[ACGT]AGG EcoRI 4154 GAATTC G^AATTC FbaI 3622 TGATCA T^GATCA Hin1I 1539 GACGTC G[GA]^CG[CT]C MseI 2728 TTAA T^TAA NaeI 40 GCCGGC GCC^GGC NdeI 4254 CATATG CA^TATG NgoMIV 40 GCCGGC G^CCGGC SfiI 1722 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1331 CACCGGTG C[GA]^CCGG[CT]G SmaI 3610 CCCGGG CCC^GGG SphI 4249 GCATGC GCATG^C TfiI 594 GATTC G^A[AT]TC Tru9I 2728 TTAA T^TAA XmaCI 3610 CCCGGG C^CCGGG XmaI 3610 CCCGGG C^CCGGG XmnI 1642 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccIII 156 TCCGGA T^CCGGA 286 TCCGGA AgeI 1332 ACCGGT A^CCGGT 1742 ACCGGT AlwI 384 GATCC GGATC(4/5) 1076 GATCC Aor51HI 1127 AGCGCT AGC^GCT 1502 AGCGCT AspI 757 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2553 GACCCTGTC AvaI 2318 CTCGGG C^[CT]CG[GA]G 3610 CCCGGG AviII 1549 TGCGCA TGC^GCA 3493 TGCGCA BglII 940 AGATCT A^GATCT 2222 AGATCT BsaI 755 GAGACC GGTCTC(1/5) 1818 GGTCTC BseAI 156 TCCGGA T^CCGGA 286 TCCGGA BsoBI 2318 CTCGGG C^[CT]CG[GA]G 3610 CCCGGG Bsp1407I 1201 TGTACA T^GTACA 1405 TGTACA BspEI 156 TCCGGA T^CCGGA 286 TCCGGA BsrGI 1201 TGTACA T^GTACA 1405 TGTACA BssHII 27 GCGCGC G^CGCGC 209 GCGCGC BssSI 2599 CTCGTG CACGAG(-5/-1) 3940 CTCGTG BstAPI 228 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1556 GCAGCTTGTGC BstEII 99 GGTGACC G^GT[ACGT]ACC 1412 GGTCACC BstPI 99 GGTGACC G^GT[ACGT]ACC 1412 GGTCACC BstYI 940 AGATCT [GA]^GATC[CT] 2222 AGATCT Eco47III 1127 AGCGCT AGC^GCT 1502 AGCGCT EcoO65I 99 GGTGACC G^GT[ACGT]ACC 1412 GGTCACC FspI 1549 TGCGCA TGC^GCA 3493 TGCGCA HindIII 990 AAGCTT A^AGCTT 1825 AAGCTT MaeII 1540 ACGT A^CGT 4002 ACGT MflI 940 AGATCT [GA]^GATC[CT] 2222 AGATCT MroI 156 TCCGGA T^CCGGA 286 TCCGGA PflFI 757 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2553 GACCCTGTC PinAI 1332 ACCGGT A^CCGGT 1742 ACCGGT PpuMI 2206 GGGACCT [GA]G^G[AT]CC[CT] 4055 GGGTCCT SalI 1542 GTCGAC G^TCGAC 3145 GTCGAC SapI 319 GAAGAGC GCTCTTC(1/4) 2811 GAAGAGC SexAI 1033 ACCTGGT A^CC[AT]GGT 4027 ACCTGGT SspBI 1201 TGTACA T^GTACA 1405 TGTACA StuI 493 AGGCCT AGG^CCT 3759 AGGCCT Tth111I 757 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2553 GACCCTGTC XhoII 940 AGATCT [GA]^GATC[CT] 2222 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwNI ApaI ApaLI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI