##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflII AflIII AscI
AseI AsnI Asp718I AvrII BamHI
BbeI BbrPI BfrI BlnI BsaAI
BsaBI BsiWI BsmBI BspDI BspHI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EcoRV
FseI HpaI KasI KpnI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SbfI
ScaI SfoI SfuI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1539 GACGTC GACGT^C
AcyI 1539 GACGTC G[GA]^CG[CT]C
AhdI 835 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3762 CCTGAGG CC^T[ACGT]AGG
Asp700I 1642 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 835 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1864 CCTCAGC CCTCAGC(-5/-2)
BcgI 1534 GCACTGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3622 TGATCA T^GATCA
BsaHI 1539 GACGTC G[GA]^CG[CT]C
BsiEI 42 CGGCCG CG[GA][CT]^CG
Bsu36I 3762 CCTGAGG CC^T[ACGT]AGG
DraIII 1205 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 42 CGGCCG C^GGCCG
Eam1105I 835 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
Eco81I 3762 CCTGAGG CC^T[ACGT]AGG
EcoRI 4154 GAATTC G^AATTC
FbaI 3622 TGATCA T^GATCA
Hin1I 1539 GACGTC G[GA]^CG[CT]C
MseI 2728 TTAA T^TAA
NaeI 40 GCCGGC GCC^GGC
NdeI 4254 CATATG CA^TATG
NgoMIV 40 GCCGGC G^CCGGC
SfiI 1722 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1331 CACCGGTG C[GA]^CCGG[CT]G
SmaI 3610 CCCGGG CCC^GGG
SphI 4249 GCATGC GCATG^C
TfiI 594 GATTC G^A[AT]TC
Tru9I 2728 TTAA T^TAA
XmaCI 3610 CCCGGG C^CCGGG
XmaI 3610 CCCGGG C^CCGGG
XmnI 1642 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 156 TCCGGA T^CCGGA
286 TCCGGA
AgeI 1332 ACCGGT A^CCGGT
1742 ACCGGT
AlwI 384 GATCC GGATC(4/5)
1076 GATCC
Aor51HI 1127 AGCGCT AGC^GCT
1502 AGCGCT
AspI 757 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2553 GACCCTGTC
AvaI 2318 CTCGGG C^[CT]CG[GA]G
3610 CCCGGG
AviII 1549 TGCGCA TGC^GCA
3493 TGCGCA
BglII 940 AGATCT A^GATCT
2222 AGATCT
BsaI 755 GAGACC GGTCTC(1/5)
1818 GGTCTC
BseAI 156 TCCGGA T^CCGGA
286 TCCGGA
BsoBI 2318 CTCGGG C^[CT]CG[GA]G
3610 CCCGGG
Bsp1407I 1201 TGTACA T^GTACA
1405 TGTACA
BspEI 156 TCCGGA T^CCGGA
286 TCCGGA
BsrGI 1201 TGTACA T^GTACA
1405 TGTACA
BssHII 27 GCGCGC G^CGCGC
209 GCGCGC
BssSI 2599 CTCGTG CACGAG(-5/-1)
3940 CTCGTG
BstAPI 228 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1556 GCAGCTTGTGC
BstEII 99 GGTGACC G^GT[ACGT]ACC
1412 GGTCACC
BstPI 99 GGTGACC G^GT[ACGT]ACC
1412 GGTCACC
BstYI 940 AGATCT [GA]^GATC[CT]
2222 AGATCT
Eco47III 1127 AGCGCT AGC^GCT
1502 AGCGCT
EcoO65I 99 GGTGACC G^GT[ACGT]ACC
1412 GGTCACC
FspI 1549 TGCGCA TGC^GCA
3493 TGCGCA
HindIII 990 AAGCTT A^AGCTT
1825 AAGCTT
MaeII 1540 ACGT A^CGT
4002 ACGT
MflI 940 AGATCT [GA]^GATC[CT]
2222 AGATCT
MroI 156 TCCGGA T^CCGGA
286 TCCGGA
PflFI 757 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2553 GACCCTGTC
PinAI 1332 ACCGGT A^CCGGT
1742 ACCGGT
PpuMI 2206 GGGACCT [GA]G^G[AT]CC[CT]
4055 GGGTCCT
SalI 1542 GTCGAC G^TCGAC
3145 GTCGAC
SapI 319 GAAGAGC GCTCTTC(1/4)
2811 GAAGAGC
SexAI 1033 ACCTGGT A^CC[AT]GGT
4027 ACCTGGT
SspBI 1201 TGTACA T^GTACA
1405 TGTACA
StuI 493 AGGCCT AGG^CCT
3759 AGGCCT
Tth111I 757 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2553 GACCCTGTC
XhoII 940 AGATCT [GA]^GATC[CT]
2222 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwNI ApaI ApaLI
ApoI AspHI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI