##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AviII BamHI BbrPI BcgI
BciVI BfrI BsaBI BseAI BsiWI
BspEI BspLU11I BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
Eam1105I EcoO65I EcoRV FseI FspI
KspI MamI MfeI MluI MroI
MunI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3554 GACGTC GACGT^C
Acc65I 3486 GGTACC G^GTACC
Aor51HI 918 AGCGCT AGC^GCT
Asp718I 3486 GGTACC G^GTACC
AvrII 2293 CCTAGG C^CTAGG
BbeI 944 GGCGCC GGCGC^C
BclI 4202 TGATCA T^GATCA
BglII 1164 AGATCT A^GATCT
BlnI 2293 CCTAGG C^CTAGG
BsaWI 435 ACCGGA [AT]^CCGG[AT]
BsmI 3381 GAATGC GAATGC(1/-1)
BspDI 2768 ATCGAT AT^CGAT
BspHI 4002 TCATGA T^CATGA
BsrFI 3949 GCCGGC [GA]^CCGG[CT]
BssSI 1443 CTCGTG CACGAG(-5/-1)
BstAPI 4583 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1164 AGATCT [GA]^GATC[CT]
Cfr10I 3949 GCCGGC [GA]^CCGG[CT]
ClaI 2768 ATCGAT AT^CGAT
DrdI 2658 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 42 CGGCCG C^GGCCG
EclXI 42 CGGCCG C^GGCCG
Eco47III 918 AGCGCT AGC^GCT
Eco52I 42 CGGCCG C^GGCCG
EcoT22I 542 ATGCAT ATGCA^T
FbaI 4202 TGATCA T^GATCA
HgaI 778 GACGC GACGC(5/10)
HindIII 3441 AAGCTT A^AGCTT
HpaI 1435 GTTAAC GTT^AAC
KasI 944 GGCGCC G^GCGCC
KpnI 3486 GGTACC GGTAC^C
MflI 1164 AGATCT [GA]^GATC[CT]
NaeI 3949 GCCGGC GCC^GGC
NarI 944 GGCGCC GG^CGCC
NdeI 4168 CATATG CA^TATG
NgoMIV 3949 GCCGGC G^CCGGC
NsiI 542 ATGCAT ATGCA^T
PaeR7I 935 CTCGAG C^TCGAG
PflMI 2077 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3333 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4002 TCATGA T^CATGA
SfoI 944 GGCGCC GGC^GCC
SphI 2036 GCATGC GCATG^C
Van91I 2077 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2893 TCTAGA T^CTAGA
XhoI 935 CTCGAG C^TCGAG
XhoII 1164 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2048 GTCTAC GT^[AC][GT]AC
3995 GTAGAC
AcyI 944 GGCGCC G[GA]^CG[CT]C
3554 GACGTC
AocI 3173 CCTCAGG CC^T[ACGT]AGG
4315 CCTGAGG
AspI 1669 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4355 GACTAAGTC
BbsI 652 GAAGAC GAAGAC(2/6)
3557 GTCTTC
BbvCI 552 CCTCAGC CCTCAGC(-5/-2)
2791 CCTCAGC
BglI 209 GCCGACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
279 GCCCCGATGGC
BpuAI 652 GAAGAC GAAGAC(2/6)
3557 GTCTTC
BsaAI 794 TACGTG [CT]AC^GT[GA]
974 TACGTG
BsaHI 944 GGCGCC G[GA]^CG[CT]C
3554 GACGTC
BsgI 869 GTGCAG GTGCAG(16/14)
2998 GTGCAG
Bsp1407I 4556 TGTACA T^GTACA
4599 TGTACA
BsrBI 37 CCGCTC CCGCTC(-3/-3)
851 GAGCGG
BsrGI 4556 TGTACA T^GTACA
4599 TGTACA
Bsu36I 3173 CCTCAGG CC^T[ACGT]AGG
4315 CCTGAGG
Eco81I 3173 CCTCAGG CC^T[ACGT]AGG
4315 CCTGAGG
EcoNI 1477 CCTGTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1490 CCTCCTGTAGG
EcoRI 478 GAATTC G^AATTC
1330 GAATTC
Hin1I 944 GGCGCC G[GA]^CG[CT]C
3554 GACGTC
NheI 1564 GCTAGC G^CTAGC
3888 GCTAGC
PflFI 1669 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4355 GACTAAGTC
SacI 305 GAGCTC GAGCT^C
494 GAGCTC
SapI 2264 GAAGAGC GCTCTTC(1/4)
307 GCTCTTC
SfiI 208 GGCCGACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
278 GGCCCCGATGGCC
SspBI 4556 TGTACA T^GTACA
4599 TGTACA
Tth111I 1669 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4355 GACTAAGTC
XcmI 2344 CCAAGCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2622 CCAGAACCTGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI