##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AvaI AvrII BamHI BbrPI
BciVI BclI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I FbaI FseI
HpaI KpnI KspI MluI MroI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI StuI SwaI
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 947 CTTAAG C^TTAAG
AhdI 2493 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 862 GGGCCC GGGCC^C
Asp700I 4326 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2493 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2762 TGCGCA TGC^GCA
BbeI 1172 GGCGCC GGCGC^C
BbsI 3122 GTCTTC GAAGAC(2/6)
BcnI 1073 CCGGG CC^[GC]GG
BfrI 947 CTTAAG C^TTAAG
BpuAI 3122 GTCTTC GAAGAC(2/6)
BsaAI 4453 TACGTG [CT]AC^GT[GA]
BsaBI 2296 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 932 TCCGGT [AT]^CCGG[AT]
BsiYI 2551 CCTTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 2551 CCTTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 3356 ACATGT A^CATGT
BspMI 198 GCAGGT ACCTGC(4/8)
BsrDI 2765 GCAATG GCAATG(2/0)
BstAPI 4057 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 3927 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2373 CCATGG C^C[GA][CT]GG
Eam1105I 2493 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2551 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4326 GAATTC G^AATTC
EcoRV 447 GATATC GAT^ATC
FspI 2762 TGCGCA TGC^GCA
HaeII 1172 GGCGCC [GA]GCGC^[CT]
HgaI 3518 GACGC GACGC(5/10)
KasI 1172 GGCGCC G^GCGCC
MamI 2296 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1172 GGCGCC GG^CGCC
NciI 1073 CCGGG CC^[GC]GG
NcoI 2373 CCATGG C^CATGG
PacI 1404 TTAATTAA TTAAT^TAA
PpuMI 4644 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 1522 GAGCTC GAGCT^C
SapI 162 GAAGAGC GCTCTTC(1/4)
SbfI 195 CCTGCAGG CCTGCA^GG
ScaI 856 AGTACT AGT^ACT
SfoI 1172 GGCGCC GGC^GCC
SphI 2686 GCATGC GCATG^C
Sse8387I 195 CCTGCAGG CCTGCA^GG
XbaI 2147 TCTAGA T^CTAGA
XmnI 4326 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1637 GTCTAC GT^[AC][GT]AC
3707 GTAGAC
AcyI 1172 GGCGCC G[GA]^CG[CT]C
3518 GACGCC
AfaI 857 GTAC GT^AC
2520 GTAC
AflIII 3356 ACATGT A^C[GA][CT]GT
4454 ACGTGT
AlwNI 1849 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
2196 CAGTTGCTG
AseI 910 ATTAAT AT^TAAT
1407 ATTAAT
AsnI 910 ATTAAT AT^TAAT
1407 ATTAAT
AspHI 1522 GAGCTC G[AT]GC[AT]^C
3691 GAGCAC
BanI 1172 GGCGCC G^G[CT][GA]CC
2958 GGTGCC
BanII 862 GGGCCC G[GA]GC[CT]^C
1522 GAGCTC
BcgI 2411 GCAGTTAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4031 GCAGAAGAATCG
BglII 1858 AGATCT A^GATCT
2152 AGATCT
BsaHI 1172 GGCGCC G[GA]^CG[CT]C
3518 GACGCC
BsiHKAI 1522 GAGCTC G[AT]GC[AT]^C
3691 GAGCAC
BssSI 1142 CACGAG CACGAG(-5/-1)
3908 CACGAG
CfoI 1173 GCGC GCG^C
2763 GCGC
DraIII 2253 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3371 CACATGGTG
EarI 162 GAAGAG CTCTTC(1/4)
2555 GAAGAG
EcoT22I 2688 ATGCAT ATGCA^T
4145 ATGCAT
FauI 231 GCGGG CCCGC(4/6)
3902 GCGGG
HapII 933 CCGG C^CGG
1073 CCGG
HhaI 1173 GCGC GCG^C
2763 GCGC
Hin1I 1172 GGCGCC G[GA]^CG[CT]C
3518 GACGCC
HinP1I 1173 GCGC G^CGC
2763 GCGC
HindIII 1904 AAGCTT A^AGCTT
2538 AAGCTT
HpaII 933 CCGG C^CGG
1073 CCGG
Ksp632I 162 GAAGAG CTCTTC(1/4)
2555 GAAGAG
MspI 933 CCGG C^CGG
1073 CCGG
NsiI 2688 ATGCAT ATGCA^T
4145 ATGCAT
PshBI 910 ATTAAT AT^TAAT
1407 ATTAAT
RsaI 857 GTAC GT^AC
2520 GTAC
SspI 874 AATATT AAT^ATT
4212 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AluI AlwI AocI
ApoI AspI AvaII BalI BbvCI
BbvI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RcaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII