################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI Asp718I AvaI AvrII BamHI BbrPI BciVI BclI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I FbaI FseI HpaI KpnI KspI MluI MroI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SspBI StuI SwaI Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflII 947 CTTAAG C^TTAAG AhdI 2493 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 862 GGGCCC GGGCC^C Asp700I 4326 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2493 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2762 TGCGCA TGC^GCA BbeI 1172 GGCGCC GGCGC^C BbsI 3122 GTCTTC GAAGAC(2/6) BcnI 1073 CCGGG CC^[GC]GG BfrI 947 CTTAAG C^TTAAG BpuAI 3122 GTCTTC GAAGAC(2/6) BsaAI 4453 TACGTG [CT]AC^GT[GA] BsaBI 2296 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 932 TCCGGT [AT]^CCGG[AT] BsiYI 2551 CCTTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 2551 CCTTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspLU11I 3356 ACATGT A^CATGT BspMI 198 GCAGGT ACCTGC(4/8) BsrDI 2765 GCAATG GCAATG(2/0) BstAPI 4057 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 3927 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2373 CCATGG C^C[GA][CT]GG Eam1105I 2493 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2551 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4326 GAATTC G^AATTC EcoRV 447 GATATC GAT^ATC FspI 2762 TGCGCA TGC^GCA HaeII 1172 GGCGCC [GA]GCGC^[CT] HgaI 3518 GACGC GACGC(5/10) KasI 1172 GGCGCC G^GCGCC MamI 2296 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1172 GGCGCC GG^CGCC NciI 1073 CCGGG CC^[GC]GG NcoI 2373 CCATGG C^CATGG PacI 1404 TTAATTAA TTAAT^TAA PpuMI 4644 AGGTCCC [GA]G^G[AT]CC[CT] SacI 1522 GAGCTC GAGCT^C SapI 162 GAAGAGC GCTCTTC(1/4) SbfI 195 CCTGCAGG CCTGCA^GG ScaI 856 AGTACT AGT^ACT SfoI 1172 GGCGCC GGC^GCC SphI 2686 GCATGC GCATG^C Sse8387I 195 CCTGCAGG CCTGCA^GG XbaI 2147 TCTAGA T^CTAGA XmnI 4326 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1637 GTCTAC GT^[AC][GT]AC 3707 GTAGAC AcyI 1172 GGCGCC G[GA]^CG[CT]C 3518 GACGCC AfaI 857 GTAC GT^AC 2520 GTAC AflIII 3356 ACATGT A^C[GA][CT]GT 4454 ACGTGT AlwNI 1849 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 2196 CAGTTGCTG AseI 910 ATTAAT AT^TAAT 1407 ATTAAT AsnI 910 ATTAAT AT^TAAT 1407 ATTAAT AspHI 1522 GAGCTC G[AT]GC[AT]^C 3691 GAGCAC BanI 1172 GGCGCC G^G[CT][GA]CC 2958 GGTGCC BanII 862 GGGCCC G[GA]GC[CT]^C 1522 GAGCTC BcgI 2411 GCAGTTAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4031 GCAGAAGAATCG BglII 1858 AGATCT A^GATCT 2152 AGATCT BsaHI 1172 GGCGCC G[GA]^CG[CT]C 3518 GACGCC BsiHKAI 1522 GAGCTC G[AT]GC[AT]^C 3691 GAGCAC BssSI 1142 CACGAG CACGAG(-5/-1) 3908 CACGAG CfoI 1173 GCGC GCG^C 2763 GCGC DraIII 2253 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG 3371 CACATGGTG EarI 162 GAAGAG CTCTTC(1/4) 2555 GAAGAG EcoT22I 2688 ATGCAT ATGCA^T 4145 ATGCAT FauI 231 GCGGG CCCGC(4/6) 3902 GCGGG HapII 933 CCGG C^CGG 1073 CCGG HhaI 1173 GCGC GCG^C 2763 GCGC Hin1I 1172 GGCGCC G[GA]^CG[CT]C 3518 GACGCC HinP1I 1173 GCGC G^CGC 2763 GCGC HindIII 1904 AAGCTT A^AGCTT 2538 AAGCTT HpaII 933 CCGG C^CGG 1073 CCGG Ksp632I 162 GAAGAG CTCTTC(1/4) 2555 GAAGAG MspI 933 CCGG C^CGG 1073 CCGG NsiI 2688 ATGCAT ATGCA^T 4145 ATGCAT PshBI 910 ATTAAT AT^TAAT 1407 ATTAAT RsaI 857 GTAC GT^AC 2520 GTAC SspI 874 AATATT AAT^ATT 4212 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AcsI AluI AlwI AocI ApoI AspI AvaII BalI BbvCI BbvI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII EaeI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PleI PstI PvuII RcaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII