##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccII AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaI AviII BbeI BbrPI
BcgI BclI BcnI BsaBI BsiEI
BsiWI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
FbaI FseI FspI HaeII KasI
KspI MamI MluI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 609 GACGTC GACGT^C
AccI 2837 GTAGAC GT^[AC][GT]AC
AccIII 476 TCCGGA T^CCGGA
AcyI 609 GACGTC G[GA]^CG[CT]C
AflII 3845 CTTAAG C^TTAAG
AocI 533 CCTGAGG CC^T[ACGT]AGG
Asp700I 227 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 399 TGGCCA TGG^CCA
BamHI 1391 GGATCC G^GATCC
BanII 3481 GAGCCC G[GA]GC[CT]^C
BbvCI 1074 CCTCAGC CCTCAGC(-5/-2)
BfrI 3845 CTTAAG C^TTAAG
BglI 2805 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4195 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4195 GCTAAGC GC^T[ACGT]AGC
BsaAI 1898 CACGTA [CT]AC^GT[GA]
BsaHI 609 GACGTC G[GA]^CG[CT]C
BsaWI 476 TCCGGA [AT]^CCGG[AT]
BseAI 476 TCCGGA T^CCGGA
BsmBI 4125 CGTCTC CGTCTC(1/5)
BspEI 476 TCCGGA T^CCGGA
BspLU11I 246 ACATGT A^CATGT
BsrFI 1664 ACCGGC [GA]^CCGG[CT]
BssSI 3554 CTCGTG CACGAG(-5/-1)
Bsu36I 533 CCTGAGG CC^T[ACGT]AGG
CelII 4195 GCTAAGC GC^T[ACGT]AGC
CfoI 4168 GCGC GCG^C
Cfr10I 1664 ACCGGC [GA]^CCGG[CT]
DsaI 4479 CCGTGG C^C[GA][CT]GG
EaeI 399 TGGCCA [CT]^GGCC[GA]
Eco81I 533 CCTGAGG CC^T[ACGT]AGG
EcoRV 3364 GATATC GAT^ATC
HgaI 880 GCGTC GACGC(5/10)
HhaI 4168 GCGC GCG^C
Hin1I 609 GACGTC G[GA]^CG[CT]C
HinP1I 4168 GCGC G^CGC
HpaI 2468 GTTAAC GTT^AAC
MfeI 1926 CAATTG C^AATTG
MluNI 399 TGGCCA TGG^CCA
MroI 476 TCCGGA T^CCGGA
MscI 399 TGGCCA TGG^CCA
MunI 1926 CAATTG C^AATTG
NheI 2974 GCTAGC G^CTAGC
PshAI 3325 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2623 CTGCAG CTGCA^G
TaqI 296 TCGA T^CGA
TthHB8I 296 TCGA T^CGA
XmnI 227 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 511 GGTACC G^GTACC
4475 GGTACC
AseI 2783 ATTAAT AT^TAAT
2932 ATTAAT
AsnI 2783 ATTAAT AT^TAAT
2932 ATTAAT
Asp718I 511 GGTACC G^GTACC
4475 GGTACC
AspHI 1779 GTGCTC G[AT]GC[AT]^C
3499 GAGCAC
AvaII 284 GGACC G^G[AT]CC
2588 GGACC
AvrII 1080 CCTAGG C^CTAGG
4466 CCTAGG
BciVI 3799 GGATAC GTATCC(6/5)
1763 GTATCC
BlnI 1080 CCTAGG C^CTAGG
4466 CCTAGG
BsaI 2897 GAGACC GGTCTC(1/5)
3652 GGTCTC
BsiHKAI 1779 GTGCTC G[AT]GC[AT]^C
3499 GAGCAC
BsmI 337 GAATGC GAATGC(1/-1)
3254 GCATTC
BspMI 1337 ACCTGC ACCTGC(4/8)
359 GCAGGT
BstAPI 1315 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3016 GCAAATTCTGC
BstXI 2305 CCATAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4500 CCAACAGTTTGG
EarI 1871 GAAGAG CTCTTC(1/4)
2378 GAAGAG
EcoNI 3753 CCTTATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4466 CCTAGGTCAGG
EcoT22I 109 ATGCAT ATGCA^T
1226 ATGCAT
FauI 2464 GCGGG CCCGC(4/6)
4069 GCGGG
HapII 477 CCGG C^CGG
1665 CCGG
HincII 2318 GTTGAC GT[CT]^[GA]AC
2468 GTTAAC
HindII 2318 GTTGAC GT[CT]^[GA]AC
2468 GTTAAC
HindIII 1468 AAGCTT A^AGCTT
2504 AAGCTT
HpaII 477 CCGG C^CGG
1665 CCGG
KpnI 511 GGTACC GGTAC^C
4475 GGTACC
Ksp632I 1871 GAAGAG CTCTTC(1/4)
2378 GAAGAG
MspA1I 1247 CAGCTG C[AC]G^C[GT]G
3287 CAGCTG
MspI 477 CCGG C^CGG
1665 CCGG
NdeI 1517 CATATG CA^TATG
4017 CATATG
NsiI 109 ATGCAT ATGCA^T
1226 ATGCAT
PflMI 963 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2656 CCAGGAAGTGG
PshBI 2783 ATTAAT AT^TAAT
2932 ATTAAT
PvuII 1247 CAGCTG CAG^CTG
3287 CAGCTG
SfcI 1094 CTATAG C^T[GA][CT]AG
2623 CTGCAG
SphI 1228 GCATGC GCATG^C
1642 GCATGC
SspI 2919 AATATT AAT^ATT
4373 AATATT
StuI 1133 AGGCCT AGG^CCT
1266 AGGCCT
Van91I 963 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2656 CCAGGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI BanI BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII