##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AvrII BclI BfrI
BglII BlnI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstZ17I ClaI
DraI Eco47III EcoRV FbaI HpaI
KpnI MluI MroI NcoI NdeI
NheI NotI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RcaI
SalI ScaI SexAI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 585 GACGTC GACGT^C
AccI 1507 GTCTAC GT^[AC][GT]AC
AflIII 3063 ACACGT A^C[GA][CT]GT
AspI 3053 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2171 TGCGCA TGC^GCA
BamHI 1610 GGATCC G^GATCC
BbeI 1386 GGCGCC GGCGC^C
BbrPI 1633 CACGTG CAC^GTG
BbvCI 2963 CCTCAGC CCTCAGC(-5/-2)
BciVI 1068 GGATAC GTATCC(6/5)
BsaBI 3149 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2396 ACCGGA [AT]^CCGG[AT]
BstAPI 1592 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3979 TTCGAA TT^CGAA
BstEII 2750 GGTGACC G^GT[ACGT]ACC
BstPI 2750 GGTGACC G^GT[ACGT]ACC
CpoI 2580 CGGACCG CG^G[AT]CCG
EcoO65I 2750 GGTGACC G^GT[ACGT]ACC
EcoRI 1462 GAATTC G^AATTC
EcoT22I 1027 ATGCAT ATGCA^T
FseI 277 GGCCGGCC GGCCGG^CC
FspI 2171 TGCGCA TGC^GCA
HindIII 1162 AAGCTT A^AGCTT
KasI 1386 GGCGCC G^GCGCC
MamI 3149 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1479 CAATTG C^AATTG
MseI 1823 TTAA T^TAA
MunI 1479 CAATTG C^AATTG
NarI 1386 GGCGCC GG^CGCC
NruI 137 TCGCGA TCG^CGA
NsiI 1027 ATGCAT ATGCA^T
NspV 3979 TTCGAA TT^CGAA
PflFI 3053 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1633 CACGTG CAC^GTG
PmlI 1633 CACGTG CAC^GTG
PvuI 140 CGATCG CGAT^CG
RsrII 2580 CGGACCG CG^G[AT]CCG
SapI 3545 GCTCTTC GCTCTTC(1/4)
SbfI 3849 CCTGCAGG CCTGCA^GG
SfiI 2413 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1386 GGCGCC GGC^GCC
SfuI 3979 TTCGAA TT^CGAA
SmlI 1798 CTCAAG C^T[CT][GA]AG
SphI 218 GCATGC GCATG^C
Sse8387I 3849 CCTGCAGG CCTGCA^GG
Tru9I 1823 TTAA T^TAA
Tth111I 3053 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1462 GAATTC [GA]^AATT[CT]
2374 AAATTC
AhdI 2978 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3153 GACATCGAGTC
AlwNI 2982 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3844 CAGGACCTG
ApoI 1462 GAATTC [GA]^AATT[CT]
2374 AAATTC
AspEI 2978 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3153 GACATCGAGTC
BfaI 3189 CTAG C^TAG
3786 CTAG
BlpI 1131 GCTCAGC GC^T[ACGT]AGC
2807 GCTCAGC
Bpu1102I 1131 GCTCAGC GC^T[ACGT]AGC
2807 GCTCAGC
BsaAI 781 TACGTG [CT]AC^GT[GA]
1633 CACGTG
BssSI 856 CACGAG CACGAG(-5/-1)
3486 CTCGTG
CelII 1131 GCTCAGC GC^T[ACGT]AGC
2807 GCTCAGC
DraIII 1258 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3347 CACAGAGTG
DrdI 2662 GACCCCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2842 GACCCCCTTGTC
Eam1105I 2978 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3153 GACATCGAGTC
EcoNI 312 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3994 CCTCTCTTAGG
HincII 1843 GTCAAC GT[CT]^[GA]AC
3059 GTCAAC
HindII 1843 GTCAAC GT[CT]^[GA]AC
3059 GTCAAC
MaeI 3189 CTAG C^TAG
3786 CTAG
NaeI 278 GCCGGC GCC^GGC
572 GCCGGC
NgoMIV 278 GCCGGC G^CCGGC
572 GCCGGC
PflMI 855 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1177 CCAGCCTGTGG
SacI 3483 GAGCTC GAGCT^C
3543 GAGCTC
TfiI 1997 GATTC G^A[AT]TC
3630 GATTC
Van91I 855 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1177 CCAGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI
XmnI