##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AflII Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AvrII
BciVI BclI BfrI BglII BlnI
BsiWI BsmI Bsp1407I BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I CpoI
DraI Eco47III EcoRV FbaI FseI
HindIII HpaI MfeI MluI MunI
NdeI NotI NspV PacI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 253 GACGTC GACGT^C
Acc65I 461 GGTACC G^GTACC
AccI 880 GTCTAC GT^[AC][GT]AC
AflIII 1550 ACGTGT A^C[GA][CT]GT
AgeI 464 ACCGGT A^CCGGT
Asp718I 461 GGTACC G^GTACC
AspI 4139 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1424 TGCGCA TGC^GCA
BbrPI 2191 CACGTG CAC^GTG
BcgI 1524 GCAGAGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2279 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2279 GCTCAGC GC^T[ACGT]AGC
BsaAI 2191 CACGTG [CT]AC^GT[GA]
BsaBI 1216 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3990 CGGCCG CG[GA][CT]^CG
BspDI 409 ATCGAT AT^CGAT
BsrDI 1108 GCAATG GCAATG(2/0)
BssHII 833 GCGCGC G^CGCGC
BstEII 1449 GGTGACC G^GT[ACGT]ACC
BstPI 1449 GGTGACC G^GT[ACGT]ACC
CelII 2279 GCTCAGC GC^T[ACGT]AGC
ClaI 409 ATCGAT AT^CGAT
DrdI 3199 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3990 CGGCCG C^GGCCG
EclXI 3990 CGGCCG C^GGCCG
Eco52I 3990 CGGCCG C^GGCCG
EcoO65I 1449 GGTGACC G^GT[ACGT]ACC
EcoRI 4289 GAATTC G^AATTC
EcoT22I 3342 ATGCAT ATGCA^T
FspI 1424 TGCGCA TGC^GCA
HincII 3331 GTTGAC GT[CT]^[GA]AC
HindII 3331 GTTGAC GT[CT]^[GA]AC
KpnI 461 GGTACC GGTAC^C
KspI 453 CCGCGG CCGC^GG
MamI 1216 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 4546 GCTAGC G^CTAGC
NruI 1283 TCGCGA TCG^CGA
NsiI 3342 ATGCAT ATGCA^T
PflFI 4139 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 464 ACCGGT A^CCGGT
PmaCI 2191 CACGTG CAC^GTG
PmlI 2191 CACGTG CAC^GTG
SacII 453 CCGCGG CCGC^GG
SapI 2692 GCTCTTC GCTCTTC(1/4)
SfiI 2258 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3344 GCATGC GCATG^C
Tth111I 4139 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 3337 TCCGGA T^CCGGA
3522 TCCGGA
AcsI 576 GAATTT [GA]^AATT[CT]
4289 GAATTC
AhdI 2125 GACTGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACCATAGGTC
ApoI 576 GAATTT [GA]^AATT[CT]
4289 GAATTC
AspEI 2125 GACTGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACCATAGGTC
BbeI 128 GGCGCC GGCGC^C
895 GGCGCC
BbsI 615 GAAGAC GAAGAC(2/6)
2720 GTCTTC
BpuAI 615 GAAGAC GAAGAC(2/6)
2720 GTCTTC
BsaI 1374 GAGACC GGTCTC(1/5)
3024 GAGACC
BseAI 3337 TCCGGA T^CCGGA
3522 TCCGGA
BsmBI 287 GAGACG CGTCTC(1/5)
536 GAGACG
BspEI 3337 TCCGGA T^CCGGA
3522 TCCGGA
DraIII 1772 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3554 CACAAGGTG
Eam1105I 2125 GACTGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACCATAGGTC
KasI 128 GGCGCC G^GCGCC
895 GGCGCC
MroI 3337 TCCGGA T^CCGGA
3522 TCCGGA
MseI 4579 TTAA T^TAA
4616 TTAA
NaeI 369 GCCGGC GCC^GGC
2840 GCCGGC
NarI 128 GGCGCC GG^CGCC
895 GGCGCC
NcoI 2383 CCATGG C^CATGG
2994 CCATGG
NgoMIV 369 GCCGGC G^CCGGC
2840 GCCGGC
PaeR7I 153 CTCGAG C^TCGAG
1998 CTCGAG
SacI 3643 GAGCTC GAGCT^C
4172 GAGCTC
SfoI 128 GGCGCC GGC^GCC
895 GGCGCC
TfiI 1756 GAATC G^A[AT]TC
4403 GATTC
Tru9I 4579 TTAA T^TAA
4616 TTAA
XhoI 153 CTCGAG C^TCGAG
1998 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI