##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AclI AflIII AgeI AocI
ApaI AscI AspI AvrII BamHI
BbrPI BbvCI BlnI BlpI Bpu1102I
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI EclXI
Eco52I Eco81I EcoO65I EcoRV FseI
HindIII HpaI MluI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 967 GACGTC GACGT^C
AccIII 473 TCCGGA T^CCGGA
AhdI 147 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1566 GTGCAC G^TGCAC
Aor51HI 168 AGCGCT AGC^GCT
ApaLI 1566 GTGCAC G^TGCAC
Asp700I 4416 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 147 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1372 TGCGCA TGC^GCA
BbeI 200 GGCGCC GGCGC^C
BcgI 4361 GCAGAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1528 TGATCA T^GATCA
BsaAI 2211 CACGTA [CT]AC^GT[GA]
BsaBI 276 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 473 TCCGGA T^CCGGA
BsiEI 214 CGGTCG CG[GA][CT]^CG
BspEI 473 TCCGGA T^CCGGA
BsrBI 267 CCGCTC CCGCTC(-3/-3)
BstAPI 4610 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2224 CCACTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 125 GACGGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 147 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 168 AGCGCT AGC^GCT
EcoNI 1637 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2390 GAATTC G^AATTC
EcoT22I 3833 ATGCAT ATGCA^T
FbaI 1528 TGATCA T^GATCA
FspI 1372 TGCGCA TGC^GCA
KasI 200 GGCGCC G^GCGCC
KspI 27 CCGCGG CCGC^GG
MamI 276 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 745 CAATTG C^AATTG
MroI 473 TCCGGA T^CCGGA
MunI 745 CAATTG C^AATTG
NarI 200 GGCGCC GG^CGCC
NcoI 1403 CCATGG C^CATGG
NdeI 496 CATATG CA^TATG
NsiI 3833 ATGCAT ATGCA^T
PaeR7I 3714 CTCGAG C^TCGAG
PpuMI 911 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1410 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 27 CCGCGG CCGC^GG
ScaI 882 AGTACT AGT^ACT
SfoI 200 GGCGCC GGC^GCC
SspI 1325 AATATT AAT^ATT
XbaI 870 TCTAGA T^CTAGA
XhoI 3714 CTCGAG C^TCGAG
XmnI 4416 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 1605 GGTACC G^GTACC
4097 GGTACC
Asp718I 1605 GGTACC G^GTACC
4097 GGTACC
BbsI 719 GAAGAC GAAGAC(2/6)
3810 GTCTTC
BciVI 1399 GTATCC GTATCC(6/5)
2921 GTATCC
BpuAI 719 GAAGAC GAAGAC(2/6)
3810 GTCTTC
BsaI 4159 GGTCTC GGTCTC(1/5)
4548 GGTCTC
BsgI 2712 CTGCAC GTGCAG(16/14)
3337 GTGCAG
BsrDI 698 CATTGC GCAATG(2/0)
4437 GCAATG
BsrFI 66 ACCGGC [GA]^CCGG[CT]
2113 GCCGGT
Cfr10I 66 ACCGGC [GA]^CCGG[CT]
2113 GCCGGT
EcoT14I 1403 CCATGG C^C[AT][AT]GG
3931 CCAAGG
FauI 266 CCCGC CCCGC(4/6)
1738 GCGGG
HaeII 168 AGCGCT [GA]GCGC^[CT]
200 GGCGCC
KpnI 1605 GGTACC GGTAC^C
4097 GGTACC
NspI 2518 GCATGT [GA]CATG^[CT]
3835 GCATGT
PstI 3121 CTGCAG CTGCA^G
3311 CTGCAG
SacI 435 GAGCTC GAGCT^C
2084 GAGCTC
StuI 1098 AGGCCT AGG^CCT
3680 AGGCCT
StyI 1403 CCATGG C^C[AT][AT]GG
3931 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BfrI BglI BglII BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII