##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AspI
BamHI BbeI BbrPI BciVI BclI
BglII BsaAI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII KasI
KpnI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PaeR7I PflFI
PmaCI PmeI PmlI PvuI RcaI
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1036 GACGTC GACGT^C
AccI 1039 GTCTAC GT^[AC][GT]AC
AclI 4442 AACGTT AA^CGTT
AflII 2226 CTTAAG C^TTAAG
AflIII 1981 ACGTGT A^C[GA][CT]GT
AgeI 791 ACCGGT A^CCGGT
AviII 2298 TGCGCA TGC^GCA
AvrII 3059 CCTAGG C^CTAGG
BcgI 2758 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2226 CTTAAG C^TTAAG
BlnI 3059 CCTAGG C^CTAGG
BsaBI 1002 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 327 GGTCTC GGTCTC(1/5)
BsaWI 791 ACCGGT [AT]^CCGG[AT]
BsmBI 708 GAGACG CGTCTC(1/5)
Bsp1407I 82 TGTACA T^GTACA
BsrFI 791 ACCGGT [GA]^CCGG[CT]
BsrGI 82 TGTACA T^GTACA
BssHII 207 GCGCGC G^CGCGC
Cfr10I 791 ACCGGT [GA]^CCGG[CT]
CpoI 103 CGGTCCG CG^G[AT]CCG
FspI 2298 TGCGCA TGC^GCA
HincII 3994 GTTAAC GT[CT]^[GA]AC
HindII 3994 GTTAAC GT[CT]^[GA]AC
HpaI 3994 GTTAAC GTT^AAC
KspI 244 CCGCGG CCGC^GG
MamI 1002 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 2975 GCTAGC G^CTAGC
PacI 4711 TTAATTAA TTAAT^TAA
PinAI 791 ACCGGT A^CCGGT
PshAI 1980 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4442 AACGTT AA^CGTT
PstI 1591 CTGCAG CTGCA^G
RsrII 103 CGGTCCG CG^G[AT]CCG
SacII 244 CCGCGG CCGC^GG
SexAI 665 ACCAGGT A^CC[AT]GGT
SphI 2301 GCATGC GCATG^C
SspBI 82 TGTACA T^GTACA
TaqI 581 TCGA T^CGA
TthHB8I 581 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AseI 4710 ATTAAT AT^TAAT
4714 ATTAAT
AsnI 4710 ATTAAT AT^TAAT
4714 ATTAAT
Asp700I 730 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1569 GAAATCCTTC
BssSI 353 CTCGTG CACGAG(-5/-1)
4486 CTCGTG
BstXI 399 CCAGCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3294 CCAAGTGCTTGG
DraI 1853 TTTAAA TTT^AAA
2395 TTTAAA
HaeII 1939 GGCGCT [GA]GCGC^[CT]
2583 GGCGCT
MslI 2597 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3358 CATCTGCATG
PshBI 4710 ATTAAT AT^TAAT
4714 ATTAAT
SapI 3257 GAAGAGC GCTCTTC(1/4)
2247 GCTCTTC
XcmI 314 CCAAGCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2889 CCAGAACTCATGTGG
XmnI 730 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1569 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmaCI XmaI