##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AclI AflII AgeI AhdI AlwNI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsiWI
Bsp1407I BspHI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MfeI MluI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tru9I Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2851 GACGTC GACGT^C
Acc65I 238 GGTACC G^GTACC
AccI 79 GTCTAC GT^[AC][GT]AC
AccIII 3835 TCCGGA T^CCGGA
Alw44I 682 GTGCAC G^TGCAC
ApaLI 682 GTGCAC G^TGCAC
Asp718I 238 GGTACC G^GTACC
BalI 4144 TGGCCA TGG^CCA
BamHI 2904 GGATCC G^GATCC
BsaBI 4286 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3835 TCCGGA T^CCGGA
BsiEI 3605 CGGTCG CG[GA][CT]^CG
BspDI 4419 ATCGAT AT^CGAT
BspEI 3835 TCCGGA T^CCGGA
BsrBI 45 GAGCGG CCGCTC(-3/-3)
BstXI 4235 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 4419 ATCGAT AT^CGAT
DraIII 1810 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 3908 GAAGAG CTCTTC(1/4)
EcoNI 4250 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4458 GAATTC G^AATTC
KpnI 238 GGTACC GGTAC^C
Ksp632I 3908 GAAGAG CTCTTC(1/4)
MamI 4286 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4144 TGGCCA TGG^CCA
MroI 3835 TCCGGA T^CCGGA
MscI 4144 TGGCCA TGG^CCA
PflMI 4591 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 3919 AGTACT AGT^ACT
SfcI 4322 CTACAG C^T[GA][CT]AG
SgrAI 422 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2946 CCCGGG CCC^GGG
SspI 4628 AATATT AAT^ATT
StuI 4183 AGGCCT AGG^CCT
Van91I 4591 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 95 TCTAGA T^CTAGA
XmaCI 2946 CCCGGG C^CCGGG
XmaI 2946 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 622 GAATTT [GA]^AATT[CT]
4458 GAATTC
Aor51HI 2700 AGCGCT AGC^GCT
3678 AGCGCT
ApoI 622 GAATTT [GA]^AATT[CT]
4458 GAATTC
BfaI 89 CTAG C^TAG
96 CTAG
BsgI 4343 CTGCAC GTGCAG(16/14)
2302 GTGCAG
BsmBI 250 GAGACG CGTCTC(1/5)
3178 GAGACG
BspLU11I 83 ACATGT A^CATGT
100 ACATGT
BstEII 3201 GGTGACC G^GT[ACGT]ACC
3426 GGTCACC
BstPI 3201 GGTGACC G^GT[ACGT]ACC
3426 GGTCACC
DrdI 3562 GACTCAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACCTCCTGGTC
Eco47III 2700 AGCGCT AGC^GCT
3678 AGCGCT
EcoO65I 3201 GGTGACC G^GT[ACGT]ACC
3426 GGTCACC
MaeI 89 CTAG C^TAG
96 CTAG
MslI 3729 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4418 CATCGATGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfiI SfoI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoI
XhoII