################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI Aor51HI AscI AseI AsnI Asp700I AspI AviII BbeI BclI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI DraIII EagI EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MluI MroI MvnI NarI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SbfI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 2679 GGTACC G^GTACC AflII 435 CTTAAG C^TTAAG AhdI 1085 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1590 GTGCAC G^TGCAC AocI 973 CCTGAGG CC^T[ACGT]AGG ApaLI 1590 GTGCAC G^TGCAC Asp718I 2679 GGTACC G^GTACC AspEI 1085 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 2679 GGTACC G^G[CT][GA]CC BbrPI 2552 CACGTG CAC^GTG BbvCI 673 GCTGAGG CCTCAGC(-5/-2) BfrI 435 CTTAAG C^TTAAG BglII 2375 AGATCT A^GATCT BsaBI 4504 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1805 GAATGC GAATGC(1/-1) Bsp1407I 651 TGTACA T^GTACA BspDI 1599 ATCGAT AT^CGAT BsrBI 4145 GAGCGG CCGCTC(-3/-3) BsrFI 4115 GCCGGC [GA]^CCGG[CT] BsrGI 651 TGTACA T^GTACA BssSI 1011 CACGAG CACGAG(-5/-1) BstAPI 2257 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 973 CCTGAGG CC^T[ACGT]AGG Cfr10I 4115 GCCGGC [GA]^CCGG[CT] ClaI 1599 ATCGAT AT^CGAT CpoI 657 CGGACCG CG^G[AT]CCG DraI 347 TTTAAA TTT^AAA Eam1105I 1085 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 973 CCTGAGG CC^T[ACGT]AGG EcoNI 4494 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 274 GATATC GAT^ATC EcoT22I 3219 ATGCAT ATGCA^T HgaI 925 GCGTC GACGC(5/10) HindIII 860 AAGCTT A^AGCTT KpnI 2679 GGTACC GGTAC^C MamI 4504 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 4115 GCCGGC GCC^GGC NdeI 1167 CATATG CA^TATG NgoMIV 4115 GCCGGC G^CCGGC NsiI 3219 ATGCAT ATGCA^T PaeR7I 3597 CTCGAG C^TCGAG PflMI 1166 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2552 CACGTG CAC^GTG PmlI 2552 CACGTG CAC^GTG RsrII 657 CGGACCG CG^G[AT]CCG SacI 3507 GAGCTC GAGCT^C SapI 3419 GAAGAGC GCTCTTC(1/4) ScaI 175 AGTACT AGT^ACT SexAI 917 ACCAGGT A^CC[AT]GGT SfiI 3076 GGCCAGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 258 ACTAGT A^CTAGT SspBI 651 TGTACA T^GTACA Van91I 1166 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 3597 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 385 GTAGAC GT^[AC][GT]AC 1362 GTAGAC ApaI 692 GGGCCC GGGCC^C 1398 GGGCCC AvrII 1102 CCTAGG C^CTAGG 2058 CCTAGG BalI 3084 TGGCCA TGG^CCA 3492 TGGCCA BcgI 1565 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2837 GCAGGTAAATCG BciVI 2341 GGATAC GTATCC(6/5) 3807 GTATCC BlnI 1102 CCTAGG C^CTAGG 2058 CCTAGG BsgI 1963 CTGCAC GTGCAG(16/14) 2861 GTGCAG BsrDI 1716 CATTGC GCAATG(2/0) 1129 GCAATG DrdI 1116 GACTCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2128 GACACAGAAGTC DsaI 3537 CCATGG C^C[GA][CT]GG 4273 CCATGG MfeI 2563 CAATTG C^AATTG 4200 CAATTG MluNI 3084 TGGCCA TGG^CCA 3492 TGGCCA MscI 3084 TGGCCA TGG^CCA 3492 TGGCCA MunI 2563 CAATTG C^AATTG 4200 CAATTG NcoI 3537 CCATGG C^CATGG 4273 CCATGG PshAI 759 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2509 GACGGGGGTC SspI 37 AATATT AAT^ATT 2609 AATATT XcmI 1256 CCACTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1330 CCATGTGTGGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BamHI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI