##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Aor51HI AscI AseI AsnI
Asp700I AspI AviII BbeI BclI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KspI
MluI MroI MvnI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SacII SalI SbfI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2679 GGTACC G^GTACC
AflII 435 CTTAAG C^TTAAG
AhdI 1085 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1590 GTGCAC G^TGCAC
AocI 973 CCTGAGG CC^T[ACGT]AGG
ApaLI 1590 GTGCAC G^TGCAC
Asp718I 2679 GGTACC G^GTACC
AspEI 1085 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2679 GGTACC G^G[CT][GA]CC
BbrPI 2552 CACGTG CAC^GTG
BbvCI 673 GCTGAGG CCTCAGC(-5/-2)
BfrI 435 CTTAAG C^TTAAG
BglII 2375 AGATCT A^GATCT
BsaBI 4504 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1805 GAATGC GAATGC(1/-1)
Bsp1407I 651 TGTACA T^GTACA
BspDI 1599 ATCGAT AT^CGAT
BsrBI 4145 GAGCGG CCGCTC(-3/-3)
BsrFI 4115 GCCGGC [GA]^CCGG[CT]
BsrGI 651 TGTACA T^GTACA
BssSI 1011 CACGAG CACGAG(-5/-1)
BstAPI 2257 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 973 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4115 GCCGGC [GA]^CCGG[CT]
ClaI 1599 ATCGAT AT^CGAT
CpoI 657 CGGACCG CG^G[AT]CCG
DraI 347 TTTAAA TTT^AAA
Eam1105I 1085 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 973 CCTGAGG CC^T[ACGT]AGG
EcoNI 4494 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 274 GATATC GAT^ATC
EcoT22I 3219 ATGCAT ATGCA^T
HgaI 925 GCGTC GACGC(5/10)
HindIII 860 AAGCTT A^AGCTT
KpnI 2679 GGTACC GGTAC^C
MamI 4504 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4115 GCCGGC GCC^GGC
NdeI 1167 CATATG CA^TATG
NgoMIV 4115 GCCGGC G^CCGGC
NsiI 3219 ATGCAT ATGCA^T
PaeR7I 3597 CTCGAG C^TCGAG
PflMI 1166 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2552 CACGTG CAC^GTG
PmlI 2552 CACGTG CAC^GTG
RsrII 657 CGGACCG CG^G[AT]CCG
SacI 3507 GAGCTC GAGCT^C
SapI 3419 GAAGAGC GCTCTTC(1/4)
ScaI 175 AGTACT AGT^ACT
SexAI 917 ACCAGGT A^CC[AT]GGT
SfiI 3076 GGCCAGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 258 ACTAGT A^CTAGT
SspBI 651 TGTACA T^GTACA
Van91I 1166 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3597 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 385 GTAGAC GT^[AC][GT]AC
1362 GTAGAC
ApaI 692 GGGCCC GGGCC^C
1398 GGGCCC
AvrII 1102 CCTAGG C^CTAGG
2058 CCTAGG
BalI 3084 TGGCCA TGG^CCA
3492 TGGCCA
BcgI 1565 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2837 GCAGGTAAATCG
BciVI 2341 GGATAC GTATCC(6/5)
3807 GTATCC
BlnI 1102 CCTAGG C^CTAGG
2058 CCTAGG
BsgI 1963 CTGCAC GTGCAG(16/14)
2861 GTGCAG
BsrDI 1716 CATTGC GCAATG(2/0)
1129 GCAATG
DrdI 1116 GACTCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACACAGAAGTC
DsaI 3537 CCATGG C^C[GA][CT]GG
4273 CCATGG
MfeI 2563 CAATTG C^AATTG
4200 CAATTG
MluNI 3084 TGGCCA TGG^CCA
3492 TGGCCA
MscI 3084 TGGCCA TGG^CCA
3492 TGGCCA
MunI 2563 CAATTG C^AATTG
4200 CAATTG
NcoI 3537 CCATGG C^CATGG
4273 CCATGG
PshAI 759 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2509 GACGGGGGTC
SspI 37 AATATT AAT^ATT
2609 AATATT
XcmI 1256 CCACTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1330 CCATGTGTGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BamHI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI