################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AviII BbeI BbrPI BbvCI BcnI BfrI BglI BsaAI BsaHI BsiEI BsiWI BsmI BspDI BsrBI BsrFI BssHII Bst1107I BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MluI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 1462 TCCGGA T^CCGGA AocI 2948 CCTAAGG CC^T[ACGT]AGG AvaI 1533 CTCGAG C^[CT]CG[GA]G BalI 3943 TGGCCA TGG^CCA BamHI 96 GGATCC G^GATCC BcgI 547 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3738 TGATCA T^GATCA BsaI 1318 GAGACC GGTCTC(1/5) BsaWI 1462 TCCGGA [AT]^CCGG[AT] BseAI 1462 TCCGGA T^CCGGA BsoBI 1533 CTCGAG C^[CT]CG[GA]G BspEI 1462 TCCGGA T^CCGGA BspHI 2534 TCATGA T^CATGA BspLU11I 1262 ACATGT A^CATGT BsrDI 4064 CATTGC GCAATG(2/0) BssSI 134 CTCGTG CACGAG(-5/-1) BstBI 2066 TTCGAA TT^CGAA Bsu36I 2948 CCTAAGG CC^T[ACGT]AGG DraIII 2929 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2948 CCTAAGG CC^T[ACGT]AGG EcoNI 3624 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2859 GAATTC G^AATTC EcoT22I 3797 ATGCAT ATGCA^T FbaI 3738 TGATCA T^GATCA HapII 1463 CCGG C^CGG HgaI 3667 GCGTC GACGC(5/10) HpaII 1463 CCGG C^CGG MluNI 3943 TGGCCA TGG^CCA MroI 1462 TCCGGA T^CCGGA MscI 3943 TGGCCA TGG^CCA MspI 1463 CCGG C^CGG NcoI 4167 CCATGG C^CATGG NsiI 3797 ATGCAT ATGCA^T NspV 2066 TTCGAA TT^CGAA PaeR7I 1533 CTCGAG C^TCGAG PflMI 1157 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1679 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2534 TCATGA T^CATGA ScaI 2122 AGTACT AGT^ACT SfuI 2066 TTCGAA TT^CGAA SmlI 1533 CTCGAG C^T[CT][GA]AG Van91I 1157 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 705 CCAGACTTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1533 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 1416 GGTACC G^GTACC 3838 GGTACC AflIII 1262 ACATGT A^C[GA][CT]GT 3298 ACGTGT AseI 936 ATTAAT AT^TAAT 959 ATTAAT AsnI 936 ATTAAT AT^TAAT 959 ATTAAT Asp718I 1416 GGTACC G^GTACC 3838 GGTACC AspHI 131 GTGCTC G[AT]GC[AT]^C 2320 GTGCTC AspI 3434 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4558 GACTTTGTC BanI 1416 GGTACC G^G[CT][GA]CC 3838 GGTACC BanII 380 GGGCTC G[GA]GC[CT]^C 2696 GAGCCC BglII 3691 AGATCT A^GATCT 4123 AGATCT BsaBI 637 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3739 GATCAAAATC BsiHKAI 131 GTGCTC G[AT]GC[AT]^C 2320 GTGCTC BsmBI 2085 CGTCTC CGTCTC(1/5) 3571 GAGACG Bsp1407I 559 TGTACA T^GTACA 4004 TGTACA BsrGI 559 TGTACA T^GTACA 4004 TGTACA BstAPI 1194 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3340 GCAGGAAATGC BstEII 2153 GGTAACC G^GT[ACGT]ACC 2214 GGTGACC BstPI 2153 GGTAACC G^GT[ACGT]ACC 2214 GGTGACC DrdI 2505 GACAGGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4027 GACTACACGGTC DsaI 385 CCACGG C^C[GA][CT]GG 4167 CCATGG EaeI 532 TGGCCG [CT]^GGCC[GA] 3943 TGGCCA EcoO65I 2153 GGTAACC G^GT[ACGT]ACC 2214 GGTGACC FauI 1958 GCGGG CCCGC(4/6) 2055 GCGGG HincII 1647 GTCAAC GT[CT]^[GA]AC 3205 GTCAAC HindII 1647 GTCAAC GT[CT]^[GA]AC 3205 GTCAAC KpnI 1416 GGTACC GGTAC^C 3838 GGTACC MamI 637 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3739 GATCAAAATC PflFI 3434 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4558 GACTTTGTC PshBI 936 ATTAAT AT^TAAT 959 ATTAAT PstI 1762 CTGCAG CTGCA^G 2966 CTGCAG SapI 1333 GAAGAGC GCTCTTC(1/4) 1551 GAAGAGC SspBI 559 TGTACA T^GTACA 4004 TGTACA TaqI 1534 TCGA T^CGA 2067 TCGA Tth111I 3434 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4558 GACTTTGTC TthHB8I 1534 TCGA T^CGA 2067 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII AvrII BbsI BbvI BciVI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XhoII XmnI