##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AviII BbeI
BbrPI BbvCI BcnI BfrI BglI
BsaAI BsaHI BsiEI BsiWI BsmI
BspDI BsrBI BsrFI BssHII Bst1107I
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KspI MluI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 1462 TCCGGA T^CCGGA
AocI 2948 CCTAAGG CC^T[ACGT]AGG
AvaI 1533 CTCGAG C^[CT]CG[GA]G
BalI 3943 TGGCCA TGG^CCA
BamHI 96 GGATCC G^GATCC
BcgI 547 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3738 TGATCA T^GATCA
BsaI 1318 GAGACC GGTCTC(1/5)
BsaWI 1462 TCCGGA [AT]^CCGG[AT]
BseAI 1462 TCCGGA T^CCGGA
BsoBI 1533 CTCGAG C^[CT]CG[GA]G
BspEI 1462 TCCGGA T^CCGGA
BspHI 2534 TCATGA T^CATGA
BspLU11I 1262 ACATGT A^CATGT
BsrDI 4064 CATTGC GCAATG(2/0)
BssSI 134 CTCGTG CACGAG(-5/-1)
BstBI 2066 TTCGAA TT^CGAA
Bsu36I 2948 CCTAAGG CC^T[ACGT]AGG
DraIII 2929 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2948 CCTAAGG CC^T[ACGT]AGG
EcoNI 3624 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2859 GAATTC G^AATTC
EcoT22I 3797 ATGCAT ATGCA^T
FbaI 3738 TGATCA T^GATCA
HapII 1463 CCGG C^CGG
HgaI 3667 GCGTC GACGC(5/10)
HpaII 1463 CCGG C^CGG
MluNI 3943 TGGCCA TGG^CCA
MroI 1462 TCCGGA T^CCGGA
MscI 3943 TGGCCA TGG^CCA
MspI 1463 CCGG C^CGG
NcoI 4167 CCATGG C^CATGG
NsiI 3797 ATGCAT ATGCA^T
NspV 2066 TTCGAA TT^CGAA
PaeR7I 1533 CTCGAG C^TCGAG
PflMI 1157 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1679 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2534 TCATGA T^CATGA
ScaI 2122 AGTACT AGT^ACT
SfuI 2066 TTCGAA TT^CGAA
SmlI 1533 CTCGAG C^T[CT][GA]AG
Van91I 1157 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 705 CCAGACTTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1533 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1416 GGTACC G^GTACC
3838 GGTACC
AflIII 1262 ACATGT A^C[GA][CT]GT
3298 ACGTGT
AseI 936 ATTAAT AT^TAAT
959 ATTAAT
AsnI 936 ATTAAT AT^TAAT
959 ATTAAT
Asp718I 1416 GGTACC G^GTACC
3838 GGTACC
AspHI 131 GTGCTC G[AT]GC[AT]^C
2320 GTGCTC
AspI 3434 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4558 GACTTTGTC
BanI 1416 GGTACC G^G[CT][GA]CC
3838 GGTACC
BanII 380 GGGCTC G[GA]GC[CT]^C
2696 GAGCCC
BglII 3691 AGATCT A^GATCT
4123 AGATCT
BsaBI 637 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3739 GATCAAAATC
BsiHKAI 131 GTGCTC G[AT]GC[AT]^C
2320 GTGCTC
BsmBI 2085 CGTCTC CGTCTC(1/5)
3571 GAGACG
Bsp1407I 559 TGTACA T^GTACA
4004 TGTACA
BsrGI 559 TGTACA T^GTACA
4004 TGTACA
BstAPI 1194 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3340 GCAGGAAATGC
BstEII 2153 GGTAACC G^GT[ACGT]ACC
2214 GGTGACC
BstPI 2153 GGTAACC G^GT[ACGT]ACC
2214 GGTGACC
DrdI 2505 GACAGGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACTACACGGTC
DsaI 385 CCACGG C^C[GA][CT]GG
4167 CCATGG
EaeI 532 TGGCCG [CT]^GGCC[GA]
3943 TGGCCA
EcoO65I 2153 GGTAACC G^GT[ACGT]ACC
2214 GGTGACC
FauI 1958 GCGGG CCCGC(4/6)
2055 GCGGG
HincII 1647 GTCAAC GT[CT]^[GA]AC
3205 GTCAAC
HindII 1647 GTCAAC GT[CT]^[GA]AC
3205 GTCAAC
KpnI 1416 GGTACC GGTAC^C
3838 GGTACC
MamI 637 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3739 GATCAAAATC
PflFI 3434 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4558 GACTTTGTC
PshBI 936 ATTAAT AT^TAAT
959 ATTAAT
PstI 1762 CTGCAG CTGCA^G
2966 CTGCAG
SapI 1333 GAAGAGC GCTCTTC(1/4)
1551 GAAGAGC
SspBI 559 TGTACA T^GTACA
4004 TGTACA
TaqI 1534 TCGA T^CGA
2067 TCGA
Tth111I 3434 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4558 GACTTTGTC
TthHB8I 1534 TCGA T^CGA
2067 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
AvrII BbsI BbvI BciVI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MunI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XhoII XmnI