##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AseI AsnI Asp700I
AspEI AspI BamHI BbrPI BclI
BfrI BglII BsaAI BsaBI BsaI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstXI BstZ17I
ClaI DrdI Eam1105I Eco47III EcoT22I
FbaI FseI HincII HindII HpaI
MamI MfeI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 894 GACGTC GACGT^C
Acc65I 3852 GGTACC G^GTACC
AflIII 3210 ACGCGT A^C[GA][CT]GT
Alw44I 3206 GTGCAC G^TGCAC
AocI 2431 CCTCAGG CC^T[ACGT]AGG
ApaLI 3206 GTGCAC G^TGCAC
AscI 1261 GGCGCGCC GG^CGCGCC
Asp718I 3852 GGTACC G^GTACC
AvrII 3860 CCTAGG C^CTAGG
BalI 3571 TGGCCA TGG^CCA
BbvCI 3662 CCTCAGC CCTCAGC(-5/-2)
BcgI 272 GCAGCTGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 718 GGATAC GTATCC(6/5)
BlnI 3860 CCTAGG C^CTAGG
Bsp1407I 2555 TGTACA T^GTACA
BspMI 3069 GCAGGT ACCTGC(4/8)
BsrDI 4172 GCAATG GCAATG(2/0)
BsrGI 2555 TGTACA T^GTACA
BstEII 1771 GGTCACC G^GT[ACGT]ACC
BstPI 1771 GGTCACC G^GT[ACGT]ACC
Bsu36I 2431 CCTCAGG CC^T[ACGT]AGG
Eco81I 2431 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1771 GGTCACC G^GT[ACGT]ACC
EcoRI 952 GAATTC G^AATTC
EcoRV 2413 GATATC GAT^ATC
HindIII 608 AAGCTT A^AGCTT
KpnI 3852 GGTACC GGTAC^C
MluI 3210 ACGCGT A^CGCGT
MluNI 3571 TGGCCA TGG^CCA
MscI 3571 TGGCCA TGG^CCA
PaeR7I 503 CTCGAG C^TCGAG
PflMI 1896 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SphI 1389 GCATGC GCATG^C
SspBI 2555 TGTACA T^GTACA
SspI 2903 AATATT AAT^ATT
Van91I 1896 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 503 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 952 GAATTC [GA]^AATT[CT]
4020 AAATTT
ApoI 952 GAATTC [GA]^AATT[CT]
4020 AAATTT
AviII 582 TGCGCA TGC^GCA
1998 TGCGCA
BsaWI 165 ACCGGA [AT]^CCGG[AT]
965 TCCGGT
BsmBI 896 CGTCTC CGTCTC(1/5)
1594 CGTCTC
BsmI 2067 GCATTC GAATGC(1/-1)
4007 GCATTC
BssSI 2707 CACGAG CACGAG(-5/-1)
713 CTCGTG
CpoI 40 CGGTCCG CG^G[AT]CCG
185 CGGACCG
EarI 3877 CTCTTC CTCTTC(1/4)
3099 GAAGAG
EcoNI 1743 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3437 CCTCATCAAGG
FspI 582 TGCGCA TGC^GCA
1998 TGCGCA
Ksp632I 3877 CTCTTC CTCTTC(1/4)
3099 GAAGAG
NaeI 1804 GCCGGC GCC^GGC
1984 GCCGGC
NgoMIV 1804 GCCGGC G^CCGGC
1984 GCCGGC
PpuMI 522 AGGACCT [GA]G^G[AT]CC[CT]
3371 GGGTCCC
RsrII 40 CGGTCCG CG^G[AT]CCG
185 CGGACCG
SgrAI 1803 CGCCGGCG C[GA]^CCGG[CT]G
1983 CGCCGGCG
SmlI 503 CTCGAG C^T[CT][GA]AG
1858 CTCAAG
TfiI 2101 GAATC G^A[AT]TC
2302 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI