##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BbrPI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FseI HincII
HindII HpaI KpnI KspI MamI
MluI NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2241 GTAGAC GT^[AC][GT]AC
AgeI 3711 ACCGGT A^CCGGT
Alw44I 1320 GTGCAC G^TGCAC
ApaLI 1320 GTGCAC G^TGCAC
Asp700I 4032 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2464 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1217 TGCGCA TGC^GCA
BbeI 1539 GGCGCC GGCGC^C
BciVI 4231 GTATCC GTATCC(6/5)
BclI 72 TGATCA T^GATCA
BspHI 798 TCATGA T^CATGA
CpoI 170 CGGACCG CG^G[AT]CCG
DraI 316 TTTAAA TTT^AAA
DraIII 48 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2536 GAAGAG CTCTTC(1/4)
EcoRI 56 GAATTC G^AATTC
EcoT14I 3798 CCATGG C^C[AT][AT]GG
FbaI 72 TGATCA T^GATCA
FspI 1217 TGCGCA TGC^GCA
KasI 1539 GGCGCC G^GCGCC
Ksp632I 2536 GAAGAG CTCTTC(1/4)
MfeI 1474 CAATTG C^AATTG
MunI 1474 CAATTG C^AATTG
NarI 1539 GGCGCC GG^CGCC
NcoI 3798 CCATGG C^CATGG
NdeI 1455 CATATG CA^TATG
PflFI 2464 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3711 ACCGGT A^CCGGT
RcaI 798 TCATGA T^CATGA
RsrII 170 CGGACCG CG^G[AT]CCG
SfoI 1539 GGCGCC GGC^GCC
StuI 977 AGGCCT AGG^CCT
StyI 3798 CCATGG C^C[AT][AT]GG
Tth111I 2464 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4032 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 56 GAATTC [GA]^AATT[CT]
1062 AAATTC
AcyI 1367 GACGCC G[GA]^CG[CT]C
1539 GGCGCC
AflIII 81 ACATGT A^C[GA][CT]GT
1412 ACATGT
AocI 2413 CCTCAGG CC^T[ACGT]AGG
2749 CCTGAGG
ApoI 56 GAATTC [GA]^AATT[CT]
1062 AAATTC
BamHI 2310 GGATCC G^GATCC
3753 GGATCC
BbsI 3766 GAAGAC GAAGAC(2/6)
2764 GTCTTC
BfaI 2315 CTAG C^TAG
4101 CTAG
BpuAI 3766 GAAGAC GAAGAC(2/6)
2764 GTCTTC
BsaHI 1367 GACGCC G[GA]^CG[CT]C
1539 GGCGCC
BsmBI 2016 GAGACG CGTCTC(1/5)
2462 GAGACG
BsmI 1376 GAATGC GAATGC(1/-1)
4032 GAATGC
BspLU11I 81 ACATGT A^CATGT
1412 ACATGT
Bsu36I 2413 CCTCAGG CC^T[ACGT]AGG
2749 CCTGAGG
Eco81I 2413 CCTCAGG CC^T[ACGT]AGG
2749 CCTGAGG
EcoNI 2452 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3400 CCTGCGGCAGG
HgaI 1367 GACGC GACGC(5/10)
3099 GACGC
Hin1I 1367 GACGCC G[GA]^CG[CT]C
1539 GGCGCC
HindIII 2503 AAGCTT A^AGCTT
2738 AAGCTT
MaeI 2315 CTAG C^TAG
4101 CTAG
MseI 317 TTAA T^TAA
1014 TTAA
NaeI 827 GCCGGC GCC^GGC
1298 GCCGGC
NgoMIV 827 GCCGGC G^CCGGC
1298 GCCGGC
SfiI 2914 GGCCGGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3184 GGCCGTGCTGGCC
SmaI 1543 CCCGGG CCC^GGG
2071 CCCGGG
SmlI 1090 CTTGAG C^T[CT][GA]AG
4204 CTCAAG
Tru9I 317 TTAA T^TAA
1014 TTAA
XmaCI 1543 CCCGGG C^CCGGG
2071 CCCGGG
XmaI 1543 CCCGGG C^CCGGG
2071 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco47III EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI TfiI TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII