################################## # NO CUT ENZYMES ( 36 enzymes) # ################################## AatII AccIII AclI AscI AvrII BclI BlnI BseAI BsiWI BspEI BstBI BstEII BstPI EcoO65I EcoRV FbaI FseI HpaI KspI MluI MroI NotI NruI NspV PacI PmeI Psp1406I PvuI SacII SalI SfuI SgrAI SnaBI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflII 2119 CTTAAG C^TTAAG AgeI 4055 ACCGGT A^CCGGT Alw44I 1630 GTGCAC G^TGCAC Aor51HI 803 AGCGCT AGC^GCT ApaLI 1630 GTGCAC G^TGCAC AseI 8953 ATTAAT AT^TAAT AsnI 8953 ATTAAT AT^TAAT BfrI 2119 CTTAAG C^TTAAG BspDI 7985 ATCGAT AT^CGAT BsrBI 8650 GAGCGG CCGCTC(-3/-3) BssHII 742 GCGCGC G^CGCGC Bst1107I 3728 GTATAC GTA^TAC BstZ17I 3728 GTATAC GTA^TAC ClaI 7985 ATCGAT AT^CGAT CpoI 10893 CGGTCCG CG^G[AT]CCG DrdI 10484 GACGCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 10889 CGGCCG C^GGCCG EclXI 10889 CGGCCG C^GGCCG Eco47III 803 AGCGCT AGC^GCT Eco52I 10889 CGGCCG C^GGCCG EcoRI 695 GAATTC G^AATTC NaeI 10887 GCCGGC GCC^GGC NgoMIV 10887 GCCGGC G^CCGGC NheI 10005 GCTAGC G^CTAGC PinAI 4055 ACCGGT A^CCGGT PshBI 8953 ATTAAT AT^TAAT RsrII 10893 CGGTCCG CG^G[AT]CCG SapI 4659 GAAGAGC GCTCTTC(1/4) SfiI 6420 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 11334 CCCGGG CCC^GGG XmaCI 11334 CCCGGG C^CCGGG XmaI 11334 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AhdI 1474 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7455 GACCCTCAGTC Asp700I 691 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 910 GAAAGTCTTC AspEI 1474 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7455 GACCCTCAGTC BamHI 3159 GGATCC G^GATCC 3500 GGATCC BciVI 4786 GGATAC GTATCC(6/5) 8571 GTATCC BglII 932 AGATCT A^GATCT 1578 AGATCT BsmBI 174 CGTCTC CGTCTC(1/5) 4531 CGTCTC Bsp1407I 2195 TGTACA T^GTACA 11390 TGTACA BspHI 1277 TCATGA T^CATGA 8030 TCATGA BspLU11I 4263 ACATGT A^CATGT 4878 ACATGT BsrGI 2195 TGTACA T^GTACA 11390 TGTACA BssSI 4943 CACGAG CACGAG(-5/-1) 11379 CTCGTG DraI 960 TTTAAA TTT^AAA 9499 TTTAAA DraIII 5038 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 7469 CACAGTGTG Eam1105I 1474 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7455 GACCCTCAGTC MfeI 4989 CAATTG C^AATTG 8989 CAATTG MunI 4989 CAATTG C^AATTG 8989 CAATTG NdeI 312 CATATG CA^TATG 1867 CATATG PaeR7I 1667 CTCGAG C^TCGAG 8581 CTCGAG PshAI 9727 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 10922 GACAGTTGTC RcaI 1277 TCATGA T^CATGA 8030 TCATGA SbfI 891 CCTGCAGG CCTGCA^GG 2439 CCTGCAGG ScaI 157 AGTACT AGT^ACT 10017 AGTACT Sse8387I 891 CCTGCAGG CCTGCA^GG 2439 CCTGCAGG SspBI 2195 TGTACA T^GTACA 11390 TGTACA XbaI 2832 TCTAGA T^CTAGA 10731 TCTAGA XhoI 1667 CTCGAG C^TCGAG 8581 CTCGAG XmnI 691 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 910 GAAAGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 162 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspHI AspI AvaI AvaII AviII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII