##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
AatII AccIII AclI AscI AvrII
BclI BlnI BseAI BsiWI BspEI
BstBI BstEII BstPI EcoO65I EcoRV
FbaI FseI HpaI KspI MluI
MroI NotI NruI NspV PacI
PmeI Psp1406I PvuI SacII SalI
SfuI SgrAI SnaBI SpeI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 2119 CTTAAG C^TTAAG
AgeI 4055 ACCGGT A^CCGGT
Alw44I 1630 GTGCAC G^TGCAC
Aor51HI 803 AGCGCT AGC^GCT
ApaLI 1630 GTGCAC G^TGCAC
AseI 8953 ATTAAT AT^TAAT
AsnI 8953 ATTAAT AT^TAAT
BfrI 2119 CTTAAG C^TTAAG
BspDI 7985 ATCGAT AT^CGAT
BsrBI 8650 GAGCGG CCGCTC(-3/-3)
BssHII 742 GCGCGC G^CGCGC
Bst1107I 3728 GTATAC GTA^TAC
BstZ17I 3728 GTATAC GTA^TAC
ClaI 7985 ATCGAT AT^CGAT
CpoI 10893 CGGTCCG CG^G[AT]CCG
DrdI 10484 GACGCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 10889 CGGCCG C^GGCCG
EclXI 10889 CGGCCG C^GGCCG
Eco47III 803 AGCGCT AGC^GCT
Eco52I 10889 CGGCCG C^GGCCG
EcoRI 695 GAATTC G^AATTC
NaeI 10887 GCCGGC GCC^GGC
NgoMIV 10887 GCCGGC G^CCGGC
NheI 10005 GCTAGC G^CTAGC
PinAI 4055 ACCGGT A^CCGGT
PshBI 8953 ATTAAT AT^TAAT
RsrII 10893 CGGTCCG CG^G[AT]CCG
SapI 4659 GAAGAGC GCTCTTC(1/4)
SfiI 6420 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 11334 CCCGGG CCC^GGG
XmaCI 11334 CCCGGG C^CCGGG
XmaI 11334 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 1474 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7455 GACCCTCAGTC
Asp700I 691 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
910 GAAAGTCTTC
AspEI 1474 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7455 GACCCTCAGTC
BamHI 3159 GGATCC G^GATCC
3500 GGATCC
BciVI 4786 GGATAC GTATCC(6/5)
8571 GTATCC
BglII 932 AGATCT A^GATCT
1578 AGATCT
BsmBI 174 CGTCTC CGTCTC(1/5)
4531 CGTCTC
Bsp1407I 2195 TGTACA T^GTACA
11390 TGTACA
BspHI 1277 TCATGA T^CATGA
8030 TCATGA
BspLU11I 4263 ACATGT A^CATGT
4878 ACATGT
BsrGI 2195 TGTACA T^GTACA
11390 TGTACA
BssSI 4943 CACGAG CACGAG(-5/-1)
11379 CTCGTG
DraI 960 TTTAAA TTT^AAA
9499 TTTAAA
DraIII 5038 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
7469 CACAGTGTG
Eam1105I 1474 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7455 GACCCTCAGTC
MfeI 4989 CAATTG C^AATTG
8989 CAATTG
MunI 4989 CAATTG C^AATTG
8989 CAATTG
NdeI 312 CATATG CA^TATG
1867 CATATG
PaeR7I 1667 CTCGAG C^TCGAG
8581 CTCGAG
PshAI 9727 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
10922 GACAGTTGTC
RcaI 1277 TCATGA T^CATGA
8030 TCATGA
SbfI 891 CCTGCAGG CCTGCA^GG
2439 CCTGCAGG
ScaI 157 AGTACT AGT^ACT
10017 AGTACT
Sse8387I 891 CCTGCAGG CCTGCA^GG
2439 CCTGCAGG
SspBI 2195 TGTACA T^GTACA
11390 TGTACA
XbaI 2832 TCTAGA T^CTAGA
10731 TCTAGA
XhoI 1667 CTCGAG C^TCGAG
8581 CTCGAG
XmnI 691 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
910 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI Asp718I
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI KpnI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII