##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BbeI BbrPI BciVI
BlnI BsiWI BspHI Bst1107I BstBI
BstXI BstZ17I CpoI Eam1105I EcoNI
EcoT22I FseI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SbfI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 2182 TCCGGA T^CCGGA
AflII 4104 CTTAAG C^TTAAG
Aor51HI 2392 AGCGCT AGC^GCT
Asp700I 1760 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 167 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1420 TGGCCA TGG^CCA
BfrI 4104 CTTAAG C^TTAAG
BglII 3433 AGATCT A^GATCT
BsaBI 1520 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2182 TCCGGA T^CCGGA
BsgI 1326 GTGCAG GTGCAG(16/14)
Bsp1407I 1612 TGTACA T^GTACA
BspDI 815 ATCGAT AT^CGAT
BspEI 2182 TCCGGA T^CCGGA
BspLU11I 1615 ACATGT A^CATGT
BsrGI 1612 TGTACA T^GTACA
BstAPI 2270 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1167 GGTGACC G^GT[ACGT]ACC
BstPI 1167 GGTGACC G^GT[ACGT]ACC
ClaI 815 ATCGAT AT^CGAT
DraI 693 TTTAAA TTT^AAA
DraIII 2271 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2964 GACCTGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2392 AGCGCT AGC^GCT
EcoO65I 1167 GGTGACC G^GT[ACGT]ACC
EcoRI 828 GAATTC G^AATTC
EcoRV 366 GATATC GAT^ATC
HindIII 1198 AAGCTT A^AGCTT
HpaI 528 GTTAAC GTT^AAC
KspI 2126 CCGCGG CCGC^GG
MamI 1520 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1420 TGGCCA TGG^CCA
MroI 2182 TCCGGA T^CCGGA
MscI 1420 TGGCCA TGG^CCA
PaeR7I 1358 CTCGAG C^TCGAG
PflFI 167 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2098 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 2126 CCGCGG CCGC^GG
SalI 3084 GTCGAC G^TCGAC
SapI 212 GAAGAGC GCTCTTC(1/4)
SexAI 2965 ACCTGGT A^CC[AT]GGT
SgrAI 2 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1612 TGTACA T^GTACA
SspI 2723 AATATT AAT^ATT
StuI 1426 AGGCCT AGG^CCT
Tth111I 167 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2098 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2836 CCACAGACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1358 CTCGAG C^TCGAG
XmnI 1760 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1320 GACGTC GACGT^C
2430 GACGTC
AcsI 828 GAATTC [GA]^AATT[CT]
3538 AAATTT
AcyI 1320 GACGTC G[GA]^CG[CT]C
2430 GACGTC
AflIII 1153 ACACGT A^C[GA][CT]GT
1615 ACATGT
AlwNI 948 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1067 CAGCATCTG
AocI 2701 CCTTAGG CC^T[ACGT]AGG
2760 CCTCAGG
ApaI 1257 GGGCCC GGGCC^C
1544 GGGCCC
ApoI 828 GAATTC [GA]^AATT[CT]
3538 AAATTT
BanI 1845 GGCACC G^G[CT][GA]CC
2547 GGTGCC
BcgI 2201 CGACTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
472 GCACTCAGTTCG
BclI 304 TGATCA T^GATCA
685 TGATCA
BfaI 2227 CTAG C^TAG
2673 CTAG
BglI 105 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1546 GCCCCCGAGGC
BsaAI 1043 CACGTA [CT]AC^GT[GA]
1154 CACGTA
BsaHI 1320 GACGTC G[GA]^CG[CT]C
2430 GACGTC
BsaI 1869 GAGACC GGTCTC(1/5)
2707 GGTCTC
BsaWI 2182 TCCGGA [AT]^CCGG[AT]
2822 TCCGGT
BsiEI 162 CGGCCG CG[GA][CT]^CG
2264 CGGCCG
BsmI 572 GAATGC GAATGC(1/-1)
2336 GCATTC
BssHII 57 GCGCGC G^CGCGC
59 GCGCGC
BssSI 1057 CACGAG CACGAG(-5/-1)
2091 CACGAG
Bsu36I 2701 CCTTAGG CC^T[ACGT]AGG
2760 CCTCAGG
EagI 162 CGGCCG C^GGCCG
2264 CGGCCG
EclXI 162 CGGCCG C^GGCCG
2264 CGGCCG
Eco52I 162 CGGCCG C^GGCCG
2264 CGGCCG
Eco81I 2701 CCTTAGG CC^T[ACGT]AGG
2760 CCTCAGG
FbaI 304 TGATCA T^GATCA
685 TGATCA
HaeII 25 GGCGCT [GA]GCGC^[CT]
2392 AGCGCT
Hin1I 1320 GACGTC G[GA]^CG[CT]C
2430 GACGTC
MaeI 2227 CTAG C^TAG
2673 CTAG
NaeI 3 GCCGGC GCC^GGC
2266 GCCGGC
NcoI 1283 CCATGG C^CATGG
1942 CCATGG
NgoMIV 3 GCCGGC G^CCGGC
2266 GCCGGC
PshAI 35 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3116 GACCCGCGTC
ScaI 2620 AGTACT AGT^ACT
2923 AGTACT
SmlI 1358 CTCGAG C^T[CT][GA]AG
4104 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AspHI AvaI AvaII AviII
BamHI BanII BbsI BbvCI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI