##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AviII
AvrII BbeI BcgI BciVI BclI
BfrI BglI BlnI BsaBI BsaWI
BseAI BspEI BsrFI BssHII BssSI
BstBI BstUI Bsu36I Cfr10I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
Psp1406I RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1270 GGTACC G^GTACC
AseI 1972 ATTAAT AT^TAAT
AsnI 1972 ATTAAT AT^TAAT
Asp718I 1270 GGTACC G^GTACC
AspI 2713 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 528 GGATCC G^GATCC
BbrPI 4508 CACGTG CAC^GTG
BlpI 1855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1855 GCTGAGC GC^T[ACGT]AGC
BsiEI 4160 CGATCG CG[GA][CT]^CG
BsiWI 2214 CGTACG C^GTACG
BsmBI 4384 GAGACG CGTCTC(1/5)
BspDI 3196 ATCGAT AT^CGAT
BspLU11I 810 ACATGT A^CATGT
BsrBI 4140 CCGCTC CCGCTC(-3/-3)
Bst1107I 517 GTATAC GTA^TAC
BstAPI 4059 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 517 GTATAC GTA^TAC
CelII 1855 GCTGAGC GC^T[ACGT]AGC
ClaI 3196 ATCGAT AT^CGAT
DraI 4524 TTTAAA TTT^AAA
DraIII 2310 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4278 CCTTGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1109 GAATTC G^AATTC
FauI 2518 CCCGC CCCGC(4/6)
HaeII 3575 AGCGCC [GA]GCGC^[CT]
KpnI 1270 GGTACC GGTAC^C
MfeI 4400 CAATTG C^AATTG
MunI 4400 CAATTG C^AATTG
PaeR7I 3179 CTCGAG C^TCGAG
PflFI 2713 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2788 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4508 CACGTG CAC^GTG
PmlI 4508 CACGTG CAC^GTG
PshAI 4245 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1972 ATTAAT AT^TAAT
PstI 3922 CTGCAG CTGCA^G
PvuI 4160 CGATCG CGAT^CG
SapI 2194 GAAGAGC GCTCTTC(1/4)
SpeI 513 ACTAGT A^CTAGT
SspI 4015 AATATT AAT^ATT
StuI 2441 AGGCCT AGG^CCT
Tth111I 2713 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2788 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3179 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 481 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
691 GAATTTGTTC
AvaI 2855 CCCGAG C^[CT]CG[GA]G
3179 CTCGAG
BalI 1434 TGGCCA TGG^CCA
1715 TGGCCA
BbvCI 2278 GCTGAGG CCTCAGC(-5/-2)
3634 GCTGAGG
BcnI 1667 CCGGG CC^[GC]GG
2490 CCGGG
BsaAI 2132 TACGTG [CT]AC^GT[GA]
4508 CACGTG
BsmI 3750 GAATGC GAATGC(1/-1)
2053 GCATTC
BsoBI 2855 CCCGAG C^[CT]CG[GA]G
3179 CTCGAG
Bsp1407I 1236 TGTACA T^GTACA
2021 TGTACA
BspHI 1419 TCATGA T^CATGA
1841 TCATGA
BsrGI 1236 TGTACA T^GTACA
2021 TGTACA
BstEII 3055 GGTTACC G^GT[ACGT]ACC
3496 GGTAACC
BstPI 3055 GGTTACC G^GT[ACGT]ACC
3496 GGTAACC
EaeI 1434 TGGCCA [CT]^GGCC[GA]
1715 TGGCCA
EcoO65I 3055 GGTTACC G^GT[ACGT]ACC
3496 GGTAACC
EcoT22I 2113 ATGCAT ATGCA^T
2484 ATGCAT
MluNI 1434 TGGCCA TGG^CCA
1715 TGGCCA
MscI 1434 TGGCCA TGG^CCA
1715 TGGCCA
NciI 1667 CCGGG CC^[GC]GG
2490 CCGGG
NdeI 788 CATATG CA^TATG
3225 CATATG
NsiI 2113 ATGCAT ATGCA^T
2484 ATGCAT
NspI 810 ACATGT [GA]CATG^[CT]
2187 GCATGT
PpuMI 1898 AGGACCT [GA]G^G[AT]CC[CT]
3701 AGGACCT
PvuII 952 CAGCTG CAG^CTG
2503 CAGCTG
RcaI 1419 TCATGA T^CATGA
1841 TCATGA
ScaI 2222 AGTACT AGT^ACT
3519 AGTACT
SfcI 3096 CTACAG C^T[GA][CT]AG
3922 CTGCAG
SspBI 1236 TGTACA T^GTACA
2021 TGTACA
XmnI 481 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
691 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII