ORK01023

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01023

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01023
Clone name	hk05446
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	TMEM127
cDNA sequence	DNA sequence (4182 bp) Predicted protein sequence (275 aa)
HaloTag ORF Clone	FHC01023
Flexi ORF Clone	FXC01023
Description	Transmembrane protein 127.

Features of the cloned cDNA sequence

Description

Length: 4182 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	3225 bp
Genome contig ID	gi89161199r_96180041
PolyA signal sequence (AATACA,-18)	+--------+--------+----*----+---- TTGTTGTTGTTAGGGAGAATACACATCTTTCTTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAGCTGGGAGTGTGTTCTCATTTCATGTCCATTCAGACAAAGCACCATTA

Features of the protein sequence

Description

Length: 275 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EDL28180	1.9e-92	96.8	transmembrane p...	Mus musculus
O75204	4.5e-89	100.0	Transmembrane p...	Homo sapiens
XP_591329	9.1e-89	99.5	similar to Tran...	Bos taurus
AAI31851	2.4e-88	99.1	Transmembrane p...	Rattus norvegicus
Q8BGP5	8.5e-88	98.7	Transmembrane p...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	69	ASALPGALSITALCTALAEPAW	90	SECONDARY	22
	2	126	TVLLLRVIAAFCFLGILCSLSAF	148	PRIMARY	23
	3	169	AHILTVLQCATVIGFSYWASELI	191	SECONDARY	23
	4	208	VTFAVSFYLVAGAGGASILATAA	230	SECONDARY	23