ORK01894

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01894

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01894
Clone name	bh00020
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	SIDT1
cDNA sequence	DNA sequence (4789 bp) Predicted protein sequence (929 aa)
HaloTag ORF Clone	FHC01894
Flexi ORF Clone	FXC01894
Description	SID1 transmembrane family member 1 precursor.

Features of the cloned cDNA sequence

Description

Length: 4789 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1866 bp
Genome contig ID	gi89161205f_114634135
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- AAAAATAAAAAAAAATAAAAATTTGAACCTGTTTC
Flanking genome sequence (196978 - 197027)	----+--------+--------+--------+--------+----* ACTATGGCTAGTTTGACCTAATTTCTTTATCTTAACGCACTATGGTTTCT

Features of the protein sequence

Description

Length: 929 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI17223	100.0	SIDT1 protein [...	Homo sapiens
XP_001158341	99.6	SID1 transmembr...	Pan troglodytes
EAW79633	99.3	SID1 transmembr...	Homo sapiens
Q9NXL6	99.2	SID1 transmembr...	Homo sapiens
XP_526266	99.0	hypothetical pr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ScanRegExp	IPR002086	5	12	PS00687	Aldehyde dehydrogenase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	100	GCLRLALLCALPWLLLAASPGHP	122	PRIMARY	23
	2	405	KSSLFSVFIFLSFYLGCLLVGFV	427	PRIMARY	23
	3	542	FWNIITIAVFYALPVIQLVITYQ	564	PRIMARY	23
	4	584	HPLGVLSAFNNILSNLGHVLLGF	606	SECONDARY	23
	5	671	DTSFMYMIAGLCMLKLYQ	688	SECONDARY	18
	6	702	YASFAVVIMVTVLGVVFGKNDVW	724	PRIMARY	23
	7	726	WVIFSAIHVLASLALSTQIYYMG	748	PRIMARY	23
	8	784	DRMVLLVVGNLVNWSFALFGLIY	806	SECONDARY	23
	9	813	SYMLGIFICNLLLYLAFYIIMK	834	PRIMARY	22
	10	843	PVPLFCIVATAVMWAAALYFFFQ	865	PRIMARY	23
	11	896	WHFLSATALFFSFLVLLTLDDDL	918	SECONDARY	23