ORK05254

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05254

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Accession No	AB208790
Product ID	ORK05254
Clone name	hk00735
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GABBR1
cDNA sequence	DNA sequence (4141 bp) Predicted protein sequence (808 aa)
Description	Gamma-aminobutyric acid type B receptor, subunit 1 precursor (GABA-B receptor 1) (GABA-B-R1) (Gb1).

Features of the cloned cDNA sequence

Description

Length: 4141 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1234 bp
Genome contig ID	gi89161210r_29577994
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- GATCTCCCCGAATCTCAATAAACCAGTGAACAGTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TGACTCGGCACCTTGCAGTCTTCCTGTGAACAGAATGGGCTTCAATCCAA

Features of the protein sequence

Description

Length: 808 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92027	100.0	gamma-aminobuty...	Homo sapiens
CAQ09396	99.7	gamma-aminobuty...	Homo sapiens
CAA09941	99.7	GABAB receptor,...	Homo sapiens
CAI18016	99.7	gamma-aminobuty...	Homo sapiens
EAX03206	99.7	gamma-aminobuty...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002456	12	33	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	32	48	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	93	121	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	98	115	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	128	147	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	190	213	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	220	243	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	230	245	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	313	331	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	317	329	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	379	402	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	437	462	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	438	461	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	477	498	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	479	500	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	510	529	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	529	546	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	561	586	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	598	618	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	647	670	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	651	673	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	678	701	PR01177	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	681	703	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002456	733	751	PR01177	Metabotropic gamma-aminobutyric acid receptor
HMMPfam	IPR001828	34	393	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR000337	442	714	PF00003	GPCR
ProfileScan	IPR000337	445	723	PS50259	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	434	LSQKLFISVSVLSSLGIVLAVVC	456	PRIMARY	23
	2	476	LNNLTAVGCSLALAAVFPLGLDG	498	SECONDARY	23
	3	561	VGLLVGMDVLTLAIWQIVDPLHR	583	SECONDARY	23
	4	619	IFYGYKGLLLLLGIFLAYETKSV	641	PRIMARY	23
	5	651	AVGMAIYNVAVLCLITAPVTMIL	673	PRIMARY	23
	6	682	AFASLAIVFSSYITLVVLFVPK	703	PRIMARY	22