ORK05436

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05436

Order Kazusa clone(s) from :

Accession No	AB209245
Product ID	ORK05436
Clone name	fj17424
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	HMGN1
cDNA sequence	DNA sequence (4260 bp) Predicted protein sequence (170 aa)
Description	Nonhistone chromosomal protein HMG-14 (High-mobility group nucleosome- binding domain-containing protein 1).

Features of the cloned cDNA sequence

Description

Length: 4260 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	487 bp
Genome contig ID	gi51511750r_39536112
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TGATCCTCAACCAATAAAATCTCAGTTATGAAAAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTTTGAAGCACATGGATTAAATTCTGGTTTAAATTTTATTTACAGAATT

Features of the protein sequence

Description

Length: 170 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92482	8.1e-77	100.0	Hypothetical pr...	Homo sapiens
EAX09655	4.4e-73	100.0	high-mobility g...	Homo sapiens
EAX09654	4.5e-73	100.0	high-mobility g...	Homo sapiens
EAX09651	4.5e-73	100.0	high-mobility g...	Homo sapiens
EAX09656	5.1e-73	100.0	high-mobility g...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	85	QRNIFINYFVNASFLVALETFL	106	SECONDARY	22
SOSUI2	2	120	KCFFLRGEIICWLFIFWYNQKIV	142	PRIMARY	23