ORK10396

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK10396

Order Kazusa clone(s) from :

Accession No
Product ID	ORK10396
Clone name	ee14717
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	FAM189B
cDNA sequence	DNA sequence (3145 bp) Predicted protein sequence (759 aa)
HaloTag ORF Clone	FHC10396
Flexi ORF Clone	FXC10396
Description	Protein COTE1

Features of the cloned cDNA sequence

Description

Length: 3145 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	576 bp
Genome contig ID	gi89161185r_153383827
PolyA signal sequence (AATAAA,-34)	+--------+--------+----*----+---- CAATAAATTTCAGTTTTCCCCTTGTTTTCTTGATC
Flanking genome sequence (99794 - 99745)	----+--------+--------+--------+--------+----* ATTCTCTGTCACCAGTGAAATTTGTTCTAGTGTCTCTCATATTTAAGAAA

Features of the protein sequence

Description

Length: 759 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH06493	99.8	Chromosome 1 op...	Homo sapiens
XP_001155325	99.7	similar to Chro...	Pan troglodytes
P81408	99.7	Protein FAM189B...	Homo sapiens
XP_001498736	96.7	hypothetical pr...	Equus caballus
XP_547550	94.9	hypothetical pr...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	126	LTLGLVQVLLGILVVTFSMVASS	148	PRIMARY	23
	2	158	SCPSWAGFSLAFSGVVGIVSW	178	SECONDARY	21
	3	186	ISFFSLLSVLCVMLSMAGSVLSC	208	PRIMARY	23
	4	268	VCGLTICAAIICTLPAIVCCIQI	290	PRIMARY	23