ORK11799

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11799

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11799
Clone name	ee18589
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	TMEM63B
cDNA sequence	DNA sequence (3162 bp) Predicted protein sequence (841 aa)
HaloTag ORF Clone	FHC11799
Flexi ORF Clone	FXC11799
Description	Transmembrane protein 63B

Features of the cloned cDNA sequence

Description

Length: 3162 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	635 bp
Genome contig ID	gi89161210f_44110273
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TTTTTCCTTTAAAATAAAAACAAAGAAAAGCTCTG
Flanking genome sequence (120962 - 121011)	----+--------+--------+--------+--------+----* AGGCAGGTGTCTGACTTGTGCTGCCCTGGGCACCTGGGCCGGGTCCCCAC

Features of the protein sequence

Description

Length: 841 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAX04247	100.0	transmembrane p...	Homo sapiens
Q5T3F8	100.0	Transmembrane p...	Homo sapiens
XP_001497713	99.0	similar to tran...	Equus caballus
XP_001929588	98.9	transmembrane p...	Sus scrofa
Q3TWI9	98.1	Transmembrane p...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003864	357	790	PF02714	Protein of unknown function DUF221

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	AAVAAMLPFLLATLGTTALNNSN	27	SECONDARY	23
	2	50	VPTVLALDFMCFLALLFLFSIL	71	PRIMARY	22
	3	167	IIGLLVVVGVLSVGIVLPVNFSG	189	PRIMARY	23
	4	211	NNLLWLHTSFAFLYLLLTVYSM	232	SECONDARY	22
	5	442	RCLVINVVLFILLFFLTTPAIII	464	PRIMARY	23
	6	488	FPTLLLWCFSALLPTIVYYSAFF	510	PRIMARY	23
	7	528	YTFLIFMVLLLPSLGLSSLDLFF	550	PRIMARY	23
	8	584	ASAFIGNAMDLLRIPGLLMYMIR	606	SECONDARY	23
	9	639	VFTVVMTYSITCPIIVPFGLMYM	661	PRIMARY	23
	10	688	GAVNQVVAAPILCLFWLLFFSTM	710	PRIMARY	23
	11	721	FTFVVLVITIVICLCHVCFGHFK	743	PRIMARY	23