ORK06304

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06304

Order Kazusa clone(s) from :

Accession No	AB209326
Product ID	ORK06304
Clone name	fh10165
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	PDE3B
cDNA sequence	DNA sequence (5521 bp) Predicted protein sequence (298 aa)
Description	cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC 3.1.4.17) (Cyclic GMP-inhibited phosphodiesterase B) (CGI-PDE B) (CGIPDE1) (CGIP1).

Features of the cloned cDNA sequence

Description

Length: 5521 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	4622 bp
Genome contig ID	gi51511727f_14522382
PolyA signal sequence (ATTAAA,-31)	+--------+--------+----*----+---- TATAATTAAAAAATAATAATAATAATAGTAATAAT
Flanking genome sequence (232405 - 232454)	----+--------+--------+--------+--------+----* AAAAAAAAAAGAAAAAAAAATTCAGACACACCTAAATGATTGTGAATGCT

Features of the protein sequence

Description

Length: 298 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92563	3e-134	100.0	phosphodiestera...	Homo sapiens
Q13370	7.6e-126	99.6	cGMP-inhibited ...	Homo sapiens
AAI46829	7.6e-126	99.6	Phosphodiestera...	synthetic construct
CAA64774	1.6e-125	99.2	cyclic nucleoti...	Homo sapiens
AAC50724	2.2e-125	99.2	cyclic nucleoti...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	17	RLSLGALAAFVLALLLG	33	PRIMARY	17
	2	50	LSVCSHSLSPLFSIACAFFFLTC	72	SECONDARY	23
	3	88	GSWWLLALPACCYLGDFLVWQWW	110	SECONDARY	23
	4	130	AAAGRLLLVLSCVGLLLTLAHPL	152	PRIMARY	23
	5	156	HCVLVLLLASFVWWVSFTSLGSL	178	PRIMARY	23