ORK10437

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK10437

Order Kazusa clone(s) from :

Accession No
Product ID	ORK10437
Clone name	pj00648
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	CSF1
cDNA sequence	DNA sequence (4062 bp) Predicted protein sequence (617 aa)
Description	Macrophage colony-stimulating factor 1 Precursor (CSF-1)(MCSF)(M-CSF)(Lanimostim) [Contains Processed macrophage colony-stimulating factor 1]

Features of the cloned cDNA sequence

Description

Length: 4062 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2154 bp
Genome contig ID	gi89161185f_110155012
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TATATATTTAATAATAAAAGAAGTGCACAAGCTGC
Flanking genome sequence (120127 - 120176)	----+--------+--------+--------+--------+----* CATGTGAGTCGTGGGAAAGTTGCTTTTGGGGTGTGGGTGGCAAACATCAG

Features of the protein sequence

Description

Length: 617 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW56417	6.3e-200	99.8	colony stimulat...	Homo sapiens
P09603	1.1e-179	99.8	Macrophage colo...	Homo sapiens
AAX36713	1.1e-179	99.8	colony stimulat...	synthetic construct
AAA59573	2.2e-179	99.8	macrophage colo...	Homo sapiens
CAH71146	2.7e-179	99.6	colony stimulat...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR008001	64	331	PF05337	Macrophage colony stimulating factor-1
HMMPfam	IPR008001	335	617	PF05337	Macrophage colony stimulating factor-1

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	75	PTTWLGSLLLLVCLLASRSITEE	97	PRIMARY	23
SOSUI2	2	558	ESVFHLLVPSVILVLLAVGGLLF	580	PRIMARY	23