ORK01014

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01014

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01014
Clone name	fj12036
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	NDC1
cDNA sequence	DNA sequence (4516 bp) Predicted protein sequence (676 aa)
HaloTag ORF Clone	FHC01014
Flexi ORF Clone	FXC01014
Description	Nucleoporin NDC1 (hNDC1) (Transmembrane protein 48).

Features of the cloned cDNA sequence

Description

Length: 4516 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2485 bp
Genome contig ID	gi89161185r_53903746
PolyA signal sequence (AATAAA,-16)	+--------+--------+----*----+---- ATACTGGATTGGGTAGAAAAATAAATTGGCAATTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAATGGAACAGTTAATTGAAATATGTGTGTTTATAAGGTCAATTAT

Features of the protein sequence

Description

Length: 676 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAX06732	100.0	transmembrane p...	Homo sapiens
Q9BTX1	100.0	Nucleoporin NDC...	Homo sapiens
AAH03082	99.8	Transmembrane p...	Homo sapiens
CAB89726	99.8	hypothetical pr...	Homo sapiens
XP_513417	98.9	hypothetical pr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	27	IVASIVWSVLFLPICTTVFIIFS	49	PRIMARY	23
	2	67	LYSSYVIFYFLLLSVVIIIISIF	89	PRIMARY	23
	3	93	FYAVVPSIPCSRLALIGKIIHPQ	115	SECONDARY	23
	4	117	LMHSFIHAAMGMVMAWCAAVITQ	139	PRIMARY	23
	5	163	CLNEYHLFFLLTGAFMGYSYSLL	185	SECONDARY	23
	6	210	SLLLLVKHSCVESLFLVRNFCIL	232	SECONDARY	23
	7	267	LNLSLLYHVWLCGVFLLTTWYVS	289	PRIMARY	23