ORK01238

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01238

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01238
Clone name	fj08903
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GABBR2
cDNA sequence	DNA sequence (3405 bp) Predicted protein sequence (950 aa)
HaloTag ORF Clone	FHC01238
Flexi ORF Clone	FXC01238
Description	gamma-aminobutyric acid (GABA) B receptor, 2

Features of the cloned cDNA sequence

Description

Length: 3405 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	289 bp
Genome contig ID	gi89161216r_99992398
PolyA signal sequence (None)	+--------+--------+----*----+---- CTGCAAACAAGGAGGCGCTGGGATATCAAACTTGC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACTAGACAAGGAGAGAGG

Features of the protein sequence

Description

Length: 950 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O75899	100.0	Gamma-aminobuty...	Homo sapiens
AAD45867	99.7	gamma-aminobuty...	Homo sapiens
AAD30389	99.8	GABA-B receptor...	Homo sapiens
AAC99345	99.7	orphan G protei...	Homo sapiens
XP_538749	99.4	similar to Gamm...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002457	44	62	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	72	88	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	83	99	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	96	113	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR000337	139	165	PR00248	GPCR
	IPR002455	144	172	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR000337	172	191	PR00248	GPCR
	IPR002455	179	198	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR000337	191	207	PR00248	GPCR
	IPR002455	241	264	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	271	294	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	299	319	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	323	342	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	344	366	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	367	379	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	376	395	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	426	445	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	451	471	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	490	513	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	529	550	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	562	581	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	581	598	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	610	635	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	647	667	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002455	696	719	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR000337	696	717	PR00248	GPCR
	IPR002455	730	752	PR01176	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	760	779	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	796	820	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	860	880	PR01178	Metabotropic gamma-aminobutyric acid receptor
	IPR002457	920	940	PR01178	Metabotropic gamma-aminobutyric acid receptor
HMMPfam	IPR001828	85	443	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR000337	494	763	PF00003	GPCR
ProfileScan	IPR000337	490	772	PS50259	GPCR
ScanRegExp	IPR000337	695	705	PS00981	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	30	ARLLLLLLLPLLLPLAPGAWGWA	52	PRIMARY	23
	2	486	ISLPLYSILSALTILGMIMASAF	508	SECONDARY	23
	3	526	PYMNNLIILGGMLSYASIFLFGL	548	SECONDARY	23
	4	561	LCTVRTWILTVGYTTAFGAMFAK	583	SECONDARY	23
	5	604	QKLLVIVGGMLLIDLCILICWQA	626	PRIMARY	23
	6	662	MTIWLGIVYAYKGLLMLFGCFLA	684	PRIMARY	23
	7	701	IGMSVYNVGIMCIIGAAVSFLT	722	PRIMARY	22
	8	731	CIVALVIIFCSTITLCLVFVPKL	753	PRIMARY	23