ORK01501

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01501

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01501
Clone name	bm02866
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	GPR62
cDNA sequence	DNA sequence (2073 bp) Predicted protein sequence (373 aa)
HaloTag ORF Clone	FHC01501
Flexi ORF Clone	FXC01501
Description	Probable G-protein coupled receptor 62 (hGPCR8).

Features of the cloned cDNA sequence

Description

Length: 2073 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	691 bp
Genome contig ID	gi89161205f_51864434
PolyA signal sequence (None)	+--------+--------+----*----+---- TGGAGGTTTTGCTGCCCACAACACCAGTATTTCAC
Flanking genome sequence (102074 - 102123)	----+--------+--------+--------+--------+----* AACTTCCTGGTCGCTGAGAATATTTATTCAAAAACAGGGATTGAAAAAAC

Features of the protein sequence

Description

Length: 373 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9BZJ7	6.9e-122	100.0	Probable G-prot...	Homo sapiens
AAH26357	2.6e-121	99.7	G protein-coupl...	Homo sapiens
NP_543141	6e-121	99.4	probable G-prot...	Homo sapiens
AAI51554	7.1e-104	90.3	GPR62 protein [...	Bos taurus
XP_850324	1.1e-100	87.6	similar to Prob...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000276	21	45	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	52	73	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	100	122	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	178	201	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	244	268	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	277	303	PR00237	Rhodopsin-like GPCR superfamily
HMMPfam	IPR000276	36	295	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	36	295	PS50262	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	21	LGLILAAVVEVGALLGNGALLVV	43	PRIMARY	23
	2	56	LAHLCVVDLLAAASIMPLGLLAA	78	PRIMARY	23
	3	96	ARFLSAALLPACTLGVAALGLAR	118	PRIMARY	23
	4	138	LTAVWAAAGLLGALSLLGPPPAP	160	SECONDARY	23
	5	183	LLAFALPALLLLGAYGGIFVVA	204	PRIMARY	22
	6	276	AEAAVTWVAYSAFAAHPFLYGLL	298	SECONDARY	23