ORK01519

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01519

Order Kazusa clone(s) from :

Accession No	AB209832
Product ID	ORK01519
Clone name	bm05234
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	DRD2
cDNA sequence	DNA sequence (2473 bp) Predicted protein sequence (453 aa)
HaloTag ORF Clone	FHC01519
Flexi ORF Clone	FXC01519
Description	D(2) dopamine receptor (Dopamine D2 receptor).

Features of the cloned cDNA sequence

Description

Length: 2473 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1111 bp
Genome contig ID	gi51511727r_112685548
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- ATCCATGCACCAAACTAATAAAACTTTGACGAGTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACCTTCCAGGACCCCTGGGTAGAAGGCAGCAGTGCCACTTCTGTGCTTGG

Features of the protein sequence

Description

Length: 453 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD93069	2e-178	100.0	dopamine recept...	Homo sapiens
EAW67225	3e-177	99.7	dopamine recept...	Homo sapiens
EAW67219	8.9e-176	98.2	dopamine recept...	Homo sapiens
BAG10953	1.6e-173	100.0	D(2) dopamine r...	synthetic construct
P14416	8.6e-173	99.7	D(2) dopamine r...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001922	13	24	PR00567	Dopamine D2 receptor
	IPR000276	47	71	PR00237	Rhodopsin-like GPCR superfamily
	IPR000929	67	77	PR00242	Dopamine receptor
	IPR000276	80	101	PR00237	Rhodopsin-like GPCR superfamily
	IPR000929	101	110	PR00242	Dopamine receptor
	IPR000276	124	146	PR00237	Rhodopsin-like GPCR superfamily
	IPR000929	145	153	PR00242	Dopamine receptor
	IPR000276	161	182	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	200	223	PR00237	Rhodopsin-like GPCR superfamily
	IPR001922	254	274	PR00567	Dopamine D2 receptor
	IPR001922	290	304	PR00567	Dopamine D2 receptor
	IPR001922	325	342	PR00567	Dopamine D2 receptor
	IPR001922	345	359	PR00567	Dopamine D2 receptor
	IPR000276	381	405	PR00237	Rhodopsin-like GPCR superfamily
	IPR001922	406	420	PR00567	Dopamine D2 receptor
	IPR000929	413	424	PR00242	Dopamine receptor
	IPR000276	418	444	PR00237	Rhodopsin-like GPCR superfamily
	IPR000929	438	452	PR00242	Dopamine receptor
HMMPfam	IPR000276	62	436	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	62	436	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	130	146	PS00237	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	50	TLLTLLIAVIVFGNVLVCMAVSR	72	PRIMARY	23
	2	82	YLIVSLAVADLLVATLVMPWVVY	104	PRIMARY	23
	3	116	HCDIFVTLDVMMCTASILNLCAI	138	SECONDARY	23
	4	162	VTVMISIVWVLSFTISCPLLFGL	184	PRIMARY	23
	5	198	AFVVYSSIVSFYVPFIVTLLVYI	220	PRIMARY	23
	6	383	QMLAIVLGVFIICWLPFFITHIL	405	PRIMARY	23
	7	419	SAFTWLGYVNSAVNPIIYTTFNI	441	SECONDARY	23