ORK01712

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01712

Order Kazusa clone(s) from :

Accession No	AB209994
Product ID	ORK01712
Clone name	bh00196
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector(Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGC TGAAGCTTTC ACAAGTTTGT ACAAAAAAGC AGGCTCTTC-5' end of cDNA, GAGCTCTCAC CACTTTGTAC AAGAAAGCTG GGCGGCCGCT TTTTTTTTTT TTTTTTT-3' end of cDNA).
Symbol	KCNC2
cDNA sequence	DNA sequence (5223 bp) Predicted protein sequence (641 aa)
HaloTag ORF Clone	FHC01712
Flexi ORF Clone	FXC01712
Description	Shaw-related voltage-gated potassium channel protein 2 isoform KV3.2c

Features of the cloned cDNA sequence

Description

Length: 5223 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	3046 bp
Genome contig ID	gi89161190r_73620125
PolyA signal sequence (TATAAA,-21)	+--------+--------+----*----+---- TAAATTACTGAAATTATAAAAAAAAAAAAAAAAAA
Flanking genome sequence (68388 - 68339)	----+--------+--------+--------+--------+----* GAAGAAGAAGAAAAAAAAAAGGGGAAGCATAAAGGTATGTAAAATAACTA

Features of the protein sequence

Description

Length: 641 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAE06076	100.0	KCNC2 variant p...	Homo sapiens
BAG10571	100.0	Shaw-related vo...	synthetic construct
Q96PR1	99.8	Potassium volta...	Homo sapiens
P22462	97.4	Potassium volta...	Rattus norvegicus
Q14B80	97.0	Potassium volta...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003968	104	114	PR01491	Kv channel
	IPR003091	104	123	PR00169	Voltage-dependent potassium channel
	IPR003974	105	118	PR01498	Shaw voltage-gated K+ channel
	IPR003974	120	135	PR01498	Shaw voltage-gated K+ channel
	IPR003974	138	159	PR01498	Shaw voltage-gated K+ channel
	IPR003974	222	234	PR01498	Shaw voltage-gated K+ channel
	IPR003091	225	253	PR00169	Voltage-dependent potassium channel
	IPR003091	287	310	PR00169	Voltage-dependent potassium channel
	IPR003091	313	333	PR00169	Voltage-dependent potassium channel
	IPR003091	355	381	PR00169	Voltage-dependent potassium channel
	IPR003968	360	368	PR01491	Kv channel
	IPR003968	384	398	PR01491	Kv channel
	IPR003091	384	407	PR00169	Voltage-dependent potassium channel
	IPR003091	424	446	PR00169	Voltage-dependent potassium channel
	IPR003091	453	479	PR00169	Voltage-dependent potassium channel
	IPR003968	464	475	PR01491	Kv channel
	IPR003974	489	503	PR01498	Shaw voltage-gated K+ channel
HMMPfam	IPR003131	13	154	PF02214	K+ channel tetramerisation
HMMPfam	IPR005821	287	475	PF00520	Ion transport
HMMSmart	IPR000210	11	162	SM00225	BTB/POZ-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	229	RAARFIAFASLFFILVSITTFCL	251	PRIMARY	23
	2	285	LTYVEGVCVVWFTFEFLVRIVFS	307	PRIMARY	23
	3	313	FIKNLLNIIDFVAILPFYLEVGL	335	PRIMARY	23
	4	378	LRASTNEFLLLIIFLALGVLIFA	400	PRIMARY	23
	5	460	CALAGVLTIAMPVPVIVNNFGMY	482	SECONDARY	23