ORK01743

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01743

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01743
Clone name	hk07376
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GPER1
cDNA sequence	DNA sequence (4162 bp) Predicted protein sequence (393 aa)
HaloTag ORF Clone	FHC01743
Flexi ORF Clone	FXC01743
Description	Chemokine receptor-like 2 (G-protein coupled receptor 30) (IL8-related receptor DRY12) (Flow-induced endothelial G-protein coupled receptor) (FEG-1) (GPCR-BR).

Features of the cloned cDNA sequence

Description

Length: 4162 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1033 bp
Genome contig ID	gi89161213f_995890
PolyA signal sequence (None)	+--------+--------+----*----+---- ACGTGGGGTGGGCATGGGGCACTCGGGAGAATCCC
Flanking genome sequence (104163 - 104212)	----+--------+--------+--------+--------+----* TCCCCCCTCACAGAAAACACAGCCCTCCCCTGCCACCCTGAAAACTCTCA

Features of the protein sequence

Description

Length: 393 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q99527	7e-158	100.0	G-protein coupl...	Homo sapiens
AAX36696	7e-158	100.0	G protein-coupl...	synthetic construct
AAC51173	1.5e-157	99.7	orphan G protei...	Homo sapiens
BAF82277	2.8e-157	99.7	unnamed protein...	Homo sapiens
CAG46456	3.8e-157	99.7	GPR30 [Homo sap...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000276	79	103	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	112	133	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	155	177	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	191	212	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	233	256	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	275	299	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	324	350	PR00237	Rhodopsin-like GPCR superfamily
HMMPfam	IPR000276	94	342	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	94	342	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	161	177	PS00237	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	78	FLSCLYTIFLFPIGFVGNILILV	100	PRIMARY	23
	2	112	PDLYFINLAVADLILVADSLIEV	134	SECONDARY	23
	3	145	AVLCTFMSLFLQVNMYSSVFFLT	167	SECONDARY	23
	4	194	LSCGLIWMASVSATLVPFTAVH	215	SECONDARY	22
	5	231	EVQWLEVTLGFIVPFAIIGLCYS	253	PRIMARY	23
	6	278	MILAVVLVFFVCWLPENVFISVH	300	PRIMARY	23
	7	325	HIVNLAAFSNSCLNPLIYSFLGE	347	SECONDARY	23