ORK01830

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01830

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01830
Clone name	ej00993
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	S1PR3
cDNA sequence	DNA sequence (4180 bp) Predicted protein sequence (399 aa)
HaloTag ORF Clone	FHC01830
Flexi ORF Clone	FXC01830
Description	Sphingosine 1-phosphate receptor Edg-3 (S1P receptor Edg-3) (Endothelial differentiation G-protein coupled receptor 3) (Sphingosine 1-phosphate receptor 3) (S1P3).

Features of the cloned cDNA sequence

Description

Length: 4180 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2666 bp
Genome contig ID	gi89161216f_90696201
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- AAAAATAATAATAATAAAACCAAAAGCCTTTGTGG
Flanking genome sequence (113539 - 113588)	----+--------+--------+--------+--------+----* AAACCCACTCTTCGTAGTCCATGTGATGGGATTGTTTTATCGTTTTCACC

Features of the protein sequence

Description

Length: 399 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q99500	4.4e-156	100.0	Sphingosine 1-p...	Homo sapiens
XP_520117	5.1e-156	99.7	endothelial dif...	Pan troglodytes
CAA58744	1.3e-155	99.7	G-protein coupl...	Homo sapiens
AAI50077	4.1e-136	88.0	S1PR3 protein [...	Bos taurus
EDL41098	1.4e-135	87.1	endothelial dif...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR004061	34	46	PR01523	Sphingosine 1-phosphate receptor
	IPR004062	45	61	PR01524	EDG-3 sphingosine 1-phosphate receptor
	IPR000276	62	86	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	95	116	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	113	122	PR01523	Sphingosine 1-phosphate receptor
	IPR004061	133	144	PR01523	Sphingosine 1-phosphate receptor
	IPR000276	139	161	PR00237	Rhodopsin-like GPCR superfamily
	IPR004062	160	176	PR01524	EDG-3 sphingosine 1-phosphate receptor
	IPR000276	174	195	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	215	225	PR01523	Sphingosine 1-phosphate receptor
	IPR000276	217	240	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	236	245	PR01523	Sphingosine 1-phosphate receptor
	IPR004062	244	254	PR01524	EDG-3 sphingosine 1-phosphate receptor
	IPR000276	262	286	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	293	304	PR01523	Sphingosine 1-phosphate receptor
	IPR000276	301	327	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	322	332	PR01523	Sphingosine 1-phosphate receptor
	IPR004062	335	344	PR01524	EDG-3 sphingosine 1-phosphate receptor
	IPR004062	347	361	PR01524	EDG-3 sphingosine 1-phosphate receptor
HMMPfam	IPR000276	77	319	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	77	319	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	145	161	PS00237	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000194	351	360	PS00152	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	7	ALSWILELIVCECQVMATALPPR	29	SECONDARY	23
	2	62	LTTVLFLVICSFIVLENLMVLIA	84	PRIMARY	23
	3	95	MYFFIGNLALCDLLAGIAYKVNI	117	SECONDARY	23
	4	138	SMFVALGASTCSLLAIAIERHLT	160	SECONDARY	23
	5	175	VFLLIGMCWLIAFTLGALPILGW	197	PRIMARY	23
	6	221	CISIFTAILVTIVILYARIYFLV	243	PRIMARY	23
	7	263	LRTVVIVVSVFIACWSPLFILFL	285	PRIMARY	23
	8	298	LFKAQWFIVLAVLNSAMNPVIYT	320	SECONDARY	23