ORK01858

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01858

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Accession No
Product ID	ORK01858
Clone name	ha02361
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of th ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
Symbol	ANAPC1
cDNA sequence	DNA sequence (7751 bp) Predicted protein sequence (1948 aa)
HaloTag ORF Clone	FHC01858
Flexi ORF Clone	FXC01858
Description	Anaphase-promoting complex subunit 1 (APC1) (Cyclosome subunit 1) (Protein Tsg24) (Mitotic checkpoint regulator).

Features of the cloned cDNA sequence

Description

Length: 7751 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1654 bp
Genome contig ID	gi89161199r_112141686
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- CTTTTCCCTGAATAAAGAATGACTGGAGAGACATT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTGTTTGCACACATATTAACTCATACCTAAGGGAGAACAGCTTTTAGA

Features of the protein sequence

Description

Length: 1948 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9H1A4	100.0	Anaphase-promot...	Homo sapiens
XP_532958	97.3	similar to anap...	Canis lupus fam...
XP_001494848	97.1	anaphase promot...	Equus caballus
XP_615671	95.5	similar to anap...	Bos taurus
XP_001250621	95.4	similar to Tsg2...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002015	1256	1283	PF01851	Proteasome/cyclosome
	IPR002015	1301	1338	PF01851	Proteasome/cyclosome
	IPR002015	1370	1396	PF01851	Proteasome/cyclosome
	IPR002015	1471	1505	PF01851	Proteasome/cyclosome

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	756	STLLFTHIPAIFFVLHLVYEELK	778	PRIMARY	23
	2	780	NTLMGEGICSLVELLVQLARDLK	802	SECONDARY	23
	3	875	RLVVLSIALYILGDESLVSDESS	897	SECONDARY	23
	4	1461	LSQAHVYIIAGACLSLGFRFAGS	1483	SECONDARY	23
	5	1514	HNLETCLSVVLLSLAMVMAGSGN	1536	PRIMARY	23