ORK01884

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01884

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01884
Clone name	fj14187
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRM4
cDNA sequence	DNA sequence (3896 bp) Predicted protein sequence (959 aa)
HaloTag ORF Clone	FHC01884
Flexi ORF Clone	FXC01884
Description	Metabotropic glutamate receptor 4 precursor (mGluR4).

Features of the cloned cDNA sequence

Description

Length: 3896 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	602 bp
Genome contig ID	gi89161210r_33998012
PolyA signal sequence (None)	+--------+--------+----*----+---- CATGTAAAATTCGGACGCGTGGGCGGACGCGTGGG
Flanking genome sequence (323023 - 322974)	----+--------+--------+--------+--------+----* GGAACAGTGAAGGAGCAGAGAGGGCGCCCCCAAACCAAAAGCCCAGAGAG

Features of the protein sequence

Description

Length: 959 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q14833	100.0	Metabotropic gl...	Homo sapiens
BAF82223	99.7	unnamed protein...	Homo sapiens
BAF82213	99.7	unnamed protein...	Homo sapiens
Q1ZZH0	99.6	Metabotropic gl...	Macaca fascicularis
XP_001928404	98.0	similar to Meta...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001786	56	73	PR01054	Metabotropic glutamate receptor 4
	IPR001786	73	91	PR01054	Metabotropic glutamate receptor 4
	IPR000337	93	105	PR00248	GPCR
	IPR000337	122	137	PR00248	GPCR
	IPR000337	137	156	PR00248	GPCR
	IPR001786	178	194	PR01054	Metabotropic glutamate receptor 4
	IPR000337	200	226	PR00248	GPCR
	IPR000337	233	252	PR00248	GPCR
	IPR000337	252	268	PR00248	GPCR
	IPR000337	268	285	PR00248	GPCR
	IPR000162	295	306	PR00593	Metabotropic glutamate receptor
	IPR000162	312	324	PR00593	Metabotropic glutamate receptor
	IPR001786	339	356	PR01054	Metabotropic glutamate receptor 4
	IPR000162	377	392	PR00593	Metabotropic glutamate receptor
	IPR001786	420	435	PR01054	Metabotropic glutamate receptor 4
	IPR000162	448	459	PR00593	Metabotropic glutamate receptor
	IPR001786	544	562	PR01054	Metabotropic glutamate receptor 4
	IPR000337	662	684	PR00248	GPCR
	IPR000162	703	717	PR00593	Metabotropic glutamate receptor
	IPR000337	707	728	PR00248	GPCR
	IPR000337	746	769	PR00248	GPCR
	IPR001786	774	788	PR01054	Metabotropic glutamate receptor 4
	IPR000337	806	829	PR00248	GPCR
	IPR000337	829	850	PR00248	GPCR
	IPR000162	868	882	PR00593	Metabotropic glutamate receptor
	IPR001786	917	931	PR01054	Metabotropic glutamate receptor 4
	IPR001786	939	958	PR01054	Metabotropic glutamate receptor 4
HMMPfam	IPR001828	124	530	PF01094	Extracellular ligand-binding receptor
	IPR011500	563	598	PF07562	Nine cysteines of 3 GPCR
	IPR000337	638	899	PF00003	GPCR
ProfileScan	IPR000337	634	908	PS50259	GPCR
ScanRegExp	IPR000337	212	230	PS00979	GPCR
	IPR000337	585	607	PS00980	GPCR
	IPR000337	828	838	PS00981	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	635	AVLPLFLAVVGIAATLFVVITFV	657	PRIMARY	23
	2	671	ELSYVLLAGIFLCYATTFLMIAE	693	SECONDARY	23
	3	744	ASQLAITFSLISLQLLGICVWFV	766	PRIMARY	23
	4	798	LSLICLLGYSMLLMVTCTVYAIK	820	PRIMARY	23
	5	836	FTMYTTCIVWLAFIPIFFGTSQS	858	PRIMARY	23
	6	866	TTTLTVSVSLSASVSLGMLYMPK	888	SECONDARY	23