ORK02116

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK02116

Order Kazusa clone(s) from :

Accession No	AB209198
Product ID	ORK02116
Clone name	fj03875
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	EDNRB
cDNA sequence	DNA sequence (4277 bp) Predicted protein sequence (459 aa)
HaloTag ORF Clone	FHC02116
Flexi ORF Clone	FXC02116
Description	Endothelin B receptor precursor (ET-B) (Endothelin receptor Non- selective type).

Features of the cloned cDNA sequence

Description

Length: 4277 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2711 bp
Genome contig ID	gi51511729r_77267625
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- TATGAAGTTATTCAATTAAAATGCCACATTTCTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TCTCTGGGCTGGACTGTGTGTTTCATTGTTGTGAAGAAACAGATATACAG

Features of the protein sequence

Description

Length: 459 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92435	2.6e-185	100.0	endothelin rece...	Homo sapiens
CAH72430	5.9e-185	99.7	endothelin rece...	Homo sapiens
XP_509693	1.2e-184	99.5	endothelin rece...	Pan troglodytes
XP_001089047	3.9e-182	97.8	endothelin rece...	Macaca mulatta
AAD24541	2.7e-181	98.4	endothelin rece...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001112	19	37	PR00571	Endothelin B receptor
	IPR001112	37	54	PR00571	Endothelin B receptor
	IPR001112	55	71	PR00571	Endothelin B receptor
	IPR001112	81	101	PR00571	Endothelin B receptor
	IPR000499	107	126	PR00366	Endothelin receptor
	IPR000276	120	144	PR00237	Rhodopsin-like GPCR superfamily
	IPR000499	141	153	PR00366	Endothelin receptor
	IPR000276	153	174	PR00237	Rhodopsin-like GPCR superfamily
	IPR001112	183	194	PR00571	Endothelin B receptor
	IPR000276	198	220	PR00237	Rhodopsin-like GPCR superfamily
	IPR000499	222	238	PR00366	Endothelin receptor
	IPR000276	234	255	PR00237	Rhodopsin-like GPCR superfamily
	IPR000499	252	266	PR00366	Endothelin receptor
	IPR001112	275	289	PR00571	Endothelin B receptor
	IPR000499	282	300	PR00366	Endothelin receptor
	IPR000276	291	314	PR00237	Rhodopsin-like GPCR superfamily
	IPR000499	306	323	PR00366	Endothelin receptor
	IPR000499	324	338	PR00366	Endothelin receptor
	IPR000276	338	362	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	385	411	PR00237	Rhodopsin-like GPCR superfamily
	IPR001112	425	443	PR00571	Endothelin B receptor
	IPR001112	444	458	PR00571	Endothelin B receptor
HMMPfam	IPR000276	135	403	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	135	403	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	204	220	PS00237	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	PSLCGRALVALVLACGLSRIWGE	44	SECONDARY	23
	2	117	FKYINTVVSCLVFVLGIIGNSTL	139	PRIMARY	23
	3	156	LIASLALGDLLHIVIDIPINVYK	178	SECONDARY	23
	4	191	CKLVPFIQKASVGITVLSLCALS	213	SECONDARY	23
	5	238	EIVLIWVVSVVLAVPEAIGFDII	260	PRIMARY	23
	6	294	LFSFYFCLPLAITAFFYTLMTCE	316	SECONDARY	23
	7	342	VFCLVLVFALCWLPLHLSRILKL	364	PRIMARY	23
	8	375	CELLSFLLVLDYIGINMASLNSC	397	SECONDARY	23